Title

Identification of target genes for ALS treatment using a vertebrate ALS model system

Alternative Title

척추 동물 모델 구축을 통한 근위축성 측색 경화증 치료 후보 유전자 발굴

DGIST Authors

Rubaiya Tabassum ; Myungin Baek ; Jaemin Lee

Advisor

백명인

Co-Advisor(s)

Jaemin Lee

Issued Date

2022

Awarded Date

2022/02

Citation

Rubaiya Tabassum. (2022). Identification of target genes for ALS treatment using a vertebrate ALS model system. doi: 10.22677/thesis.200000592213

Type

Thesis

Subject

Brain & Cognitive Sciences

Description

Brain & Cognitive Sciences

Table Of Contents

I. INTRODUCTION 1
1.1 Cell death in SOD1G93A-linked ALS 1
1.2 Cell death in TDP43Q331K-linked ALS 1
1.3 Cell death in C9Orf72PR50-linked ALS 2
1.4 Current treatment for ALS 3
II. MATERIALS AND METHODS 6
2.1 Construction of expression vectors 6
2.2 In ovo electroporation 6
2.3 Tissue preparation for immunostaining 6
2.4 RNA In situ Hybridization 7
2.5 Immunohistochemistry and confocal Imaging 8
2.6 Image and Statistical analysis 8
III. RESULTS 10
3.1 Embryonic chicken as ALS model and mRNA expression in spinal cord neuron 10
3.2 mRNA expression in Tfam electroporated spinal cord 11
3.3 mRNA expression in Gpld1 electroporated spinal cord 12
3.4 Caspase-3 positive cells in different ALS mutants 15
3.5 Protective effects of Tfam in ALS mutants 15
3.6 Protective effects of Gpld1 in ALS mutants 18
IV. DISCUSSION 19
V. CONCLUSION 21
VI. REFERENCES 22
요약문 27

URI

http://dgist.dcollection.net/common/orgView/200000592213
http://hdl.handle.net/20.500.11750/16289

DOI

10.22677/thesis.200000592213

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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