DGIST Scholar: Single-cell RNA sequencing reveals that Baf contributes to neural progenitor competence of Drosophila nervous system in a lineage-specific manner

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Single-cell RNA sequencing reveals that Baf contributes to neural progenitor competence of Drosophila nervous system in a lineage-specific manner

Title: Single-cell RNA sequencing reveals that Baf contributes to neural progenitor competence of Drosophila nervous system in a lineage-specific manner

Alternative Title: 단일 세포 전사체 분석을 통한 Drosophila 신경계 내에서 Baf가 계통특이적 신경전구세포 분화능에 미치는 영향에 관한 연구

Subject: scRNA-seq, Barrier to autointegration factor (Baf), chromatin organization, neural progenitor competence, neurodevelopment

Description: scRNA-seq, Barrier to autointegration factor (Baf), chromatin organization, neural progenitor competence, neurodevelopment

Table Of Contents: I. Introduction 1
II. Preliminaries 3
2.1 Drosophila neuroblasts with Baf knockdown shows abnormal chromatin organization and formation of neuroblast clones 3
2.2 pan-neuronal knockdown of Baf induces marked reduction of excitatory neurons at the adult stage 3
2.3 Neuronal knockdown of Baf induces behavioral abnormalities at the adult stage 4
III. Results 5
3.1 scRNA-seq identifies cell types in neuronal lineage from control and Baf KD Drosophila 5
3.2 Baf knockdown induces transcriptional changes in Type I neuroblasts 7
3.3 Type I neuroblast-specific neurodevelopment is impaired by Baf knockdown 8
IV. Discussion 11
V. Materials and methods 13
5.1 Sample preparation for single-cell sequencing 13
5.2 scRNA-seq data preprocessing 13
5.3 scRNA-seq data analysis 14
References 15
요약문 20

URI: http://dgist.dcollection.net/common/orgView/200000591876

http://hdl.handle.net/20.500.11750/16326

Related Researcher

Kwak, June Myoung 뉴바이올로지학과
Research Interests Calcium Signaling; ABA;ROS Signaling; plant development; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation

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