Title

Single-cell RNA sequencing reveals that Baf contributes to neural progenitor competence of Drosophila nervous system in a lineage-specific manner

Alternative Title

단일 세포 전사체 분석을 통한 Drosophila 신경계 내에서 Baf가 계통특이적 신경전구세포 분화능에 미치는 영향에 관한 연구

DGIST Authors

Dong Gon Cha ; Jong Kyoung Kim ; June M. Kwak

Advisor

김종경

Co-Advisor(s)

June M. Kwak

Issued Date

2022

Awarded Date

2022/02

Citation

Dong Gon Cha. (2022). Single-cell RNA sequencing reveals that Baf contributes to neural progenitor competence of Drosophila nervous system in a lineage-specific manner. doi: 10.22677/thesis.200000591876

Type

Thesis

Subject

scRNA-seq, Barrier to autointegration factor (Baf), chromatin organization, neural progenitor competence, neurodevelopment

Description

scRNA-seq, Barrier to autointegration factor (Baf), chromatin organization, neural progenitor competence, neurodevelopment

Table Of Contents

I. Introduction 1
II. Preliminaries 3
2.1 Drosophila neuroblasts with Baf knockdown shows abnormal chromatin organization and formation of neuroblast clones 3
2.2 pan-neuronal knockdown of Baf induces marked reduction of excitatory neurons at the adult stage 3
2.3 Neuronal knockdown of Baf induces behavioral abnormalities at the adult stage 4
III. Results 5
3.1 scRNA-seq identifies cell types in neuronal lineage from control and Baf KD Drosophila 5
3.2 Baf knockdown induces transcriptional changes in Type I neuroblasts 7
3.3 Type I neuroblast-specific neurodevelopment is impaired by Baf knockdown 8
IV. Discussion 11
V. Materials and methods 13
5.1 Sample preparation for single-cell sequencing 13
5.2 scRNA-seq data preprocessing 13
5.3 scRNA-seq data analysis 14
References 15
요약문 20

URI

http://dgist.dcollection.net/common/orgView/200000591876
http://hdl.handle.net/20.500.11750/16326

DOI

10.22677/thesis.200000591876

Degree

Master

Department

New Biology

Publisher

DGIST

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