Dong Gon Cha. (2022). Single-cell RNA sequencing reveals that Baf contributes to neural progenitor competence of Drosophila nervous system in a lineage-specific manner. doi: 10.22677/thesis.200000591876
I. Introduction 1 II. Preliminaries 3 2.1 Drosophila neuroblasts with Baf knockdown shows abnormal chromatin organization and formation of neuroblast clones 3 2.2 pan-neuronal knockdown of Baf induces marked reduction of excitatory neurons at the adult stage 3 2.3 Neuronal knockdown of Baf induces behavioral abnormalities at the adult stage 4 III. Results 5 3.1 scRNA-seq identifies cell types in neuronal lineage from control and Baf KD Drosophila 5 3.2 Baf knockdown induces transcriptional changes in Type I neuroblasts 7 3.3 Type I neuroblast-specific neurodevelopment is impaired by Baf knockdown 8 IV. Discussion 11 V. Materials and methods 13 5.1 Sample preparation for single-cell sequencing 13 5.2 scRNA-seq data preprocessing 13 5.3 scRNA-seq data analysis 14 References 15 요약문 20
