Title

Strategy for identifying organophosphate adducts in biological samples using high resolution mass spectrometry

DGIST Authors

Jun Hyung Lee ; Min-Sik Kim ; Jin Hae Kim

Advisor

김민식

Co-Advisor(s)

Jin Hae Kim

Issued Date

2022

Awarded Date

2022/02

Citation

Jun Hyung Lee. (2022). Strategy for identifying organophosphate adducts in biological samples using high resolution mass spectrometry. doi: 10.22677/thesis.200000596601

Type

Thesis

Subject

Mass spectrometry, Human Serum Albumin, Organophosphate, Nuerotoxicity, Open-search

Description

Mass spectrometry, Human Serum Albumin, Organophosphate, Nuerotoxicity, Open-search

Table Of Contents

Ⅰ. INTRODUCTION 1
1.1 Incidents of nerve agent exposure 1
1.2 Organophosphate 1
1.3 Etiology 2
1.4 Pathophysiology & Toxicokinetics 2
1.5 Detection method for Organophosphate 2
1.6 Human Serum Albumin (HSA) 3
1.7 Proteomics 3
Ⅱ. Material and Method 4
2.1 Materials 4
2.2 Sample preparation method 5
2.3 Q-Exactive LC-MS-MS analysis 5
2.4 Data analysis 5
2.5 Crystal structure of human albumin visualization 6
Ⅲ. Result & Discussion 6
3.1 OP-HSA modification site detection 6
3.2 Characteristic of potential binding affinity at OP-modification site 7
3.3 Y263 site have a immonium ion-fragmentation characteristic 7
3.4 IM-based search method 8
3.5 Discussion 8
Reference 19
요약문 21

URI

http://dgist.dcollection.net/common/orgView/200000596601
http://hdl.handle.net/20.500.11750/16327

DOI

10.22677/thesis.200000596601

Degree

Master

Department

New Biology

Publisher

DGIST

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