Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이성배 | - |
| dc.contributor.author | In Jun Cha | - |
| dc.date.accessioned | 2022-07-07T02:29:09Z | - |
| dc.date.available | 2022-07-07T02:29:09Z | - |
| dc.date.issued | 2021 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000363192 | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/16676 | - |
| dc.description.statementofresponsibility | N | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 ⅠⅠ. MATERIALS AND METHODS 4 2-1. Fly stocks 4 2-2. Generation of transgenic fly lines 5 2-3. Microscope image acquisition 5 2-4. Immunostaining 6 2-5. Image processing and analyses 6 2-6. Rapid Iterative Negative Geotaxis (RING) assay 7 2-7. Separation of SDS–soluble and –insoluble fractions for Western blot analysis 7 2-8. Nuclear extraction 8 2-9. Western blot 8 2-10. Statistical analysis 9 2-11. Analysis of survival rate 9 2-12. Identification of Q-rich proteins 9 2-13. Calculation of co-localization score (CLS) 10 2-14. Calculation of cytoplasm to nucleus score (CNS) 10 2-15. Partial least square-discriminatory analysis (PLS-DA) 11 2-16. Functional enrichment analysis 12 2-17. Reconstruction of network model 13 ⅠⅠⅠ. RESULTS 14 3-1. Conversion of cytoplasmic mHtt to cleaved nuclear mHtt is critical for mHtt toxicity 14 3-2. Knockdown of strong mHtt interactors reduces cytoplasmic mHtt aggregates 25 3-3. Cytoplasmic localization and Q-richness are important for strong interaction with cytoplasmic mHtt proteins 46 3-4. In silico model predicts cytoskeletal regulators as strong interactors to modulate the amounts of cytoplasmic mHtt aggregates 56 3-5. Knockdown of strong mHtt interactors increases mHtt toxicity in neurons 62 ⅠV. DISCUSSION 74 V. REFERENCES 78 VI. SUMMARY IN KOREAN 88 |
- |
| dc.format.extent | 89 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.subject | Huntington’s disease, Huntingtin, Cytoplasmic aggregates, Neuronal toxicity, 헌팅턴씨 병, 헌팅틴 단백질, 세포질 응집체, 신경세포 독성 | - |
| dc.title | Glutamine-rich interactors of mutant huntingtin modulate neuronal toxicity by promoting cytoplasmic huntingtin aggregation | - |
| dc.title.alternative | 독성 헌팅틴 단백질과 상호작용하는 글루타민 풍부 단백질의 세포질 헌팅틴 응집체 형성 촉진 및 신경세포 독성 조절 이해 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/thesis.200000363192 | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | Brain and Cognitive Sciences | - |
| dc.contributor.coadvisor | Daehee Hwang | - |
| dc.date.awarded | 2021/02 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.BD 차68 202102 | - |
| dc.contributor.alternativeDepartment | 뇌인지과학전공 | - |
| dc.embargo.liftdate | 2023-02-28 | - |
| dc.contributor.affiliatedAuthor | In Jun Cha | - |
| dc.contributor.affiliatedAuthor | Sung Bae Lee | - |
| dc.contributor.affiliatedAuthor | Daehee Hwang | - |
| dc.contributor.alternativeName | 차인준 | - |
| dc.contributor.alternativeName | Sung Bae Lee | - |
| dc.contributor.alternativeName | 황대희 | - |
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