Title

Active construction of neural network using magnetically actuated microrobots for selective signal transmission

Alternative Title

신경 네트워크 형성을 위한 나노구조 표면 및 마이크로로봇 개발

DGIST Authors

Eunhee Kim ; Hongsoo Choi ; Seong-Woon Yu

Advisor

최홍수

Co-Advisor(s)

Seong-Woon Yu

Issued Date

2021

Awarded Date

2021/02

Citation

Eunhee Kim. (2021). Active construction of neural network using magnetically actuated microrobots for selective signal transmission. doi: 10.22677/thesis.200000363961

Type

Thesis

Subject

Nanostructured surface, Neural network, Microrobot, Targeted therapy, Microelectrode array (MEA), 나노구조 표면, 신경 네트워크, 마이크로로봇, 표적 치료, 미세 전극 어레이

Table Of Contents

1. INTRODUCTION 1
1.1 Background 1
1.1.1 Biomedical microrobots 1
1.1.2 In vitro neural network 6
1.2 Research trend or related research works 12
1.2.1 Microrobots for cell delivery 12
1.2.2 Engineered networks for structural and functional connection 18
1.2.3 Multielectrode arrays (MEAs) 21
1.3 Aims of the current study 26
2. NANO-PATTERNED SU-8 SURFACE USING NANOSPHERE-LITHOGRAPHY FOR ENHANCED NEURONAL CELL GROWTH 27
2.1 Introduction 27
2.2 Materials and methods 30
2.3 Results and discussion 35
2.4 Conclusion 48
3. AN SU-8-BASED MICROPROBE WITH A NANOSTRUCTURED SURFACE ENHANCES NEURONAL CELL ATTACHMENT AND GROWTH 49
3.1 Introduction 49
3.2 Materials and methods 52
3.3 Results and discussion 56
3.4 Conclusion 63
4. A MAGNETICALLY ACTUATED MICROROBOT FOR TARGETED NEURAL CELL DELIVERY AND SELECTIVE CONNECTION OF NEURAL NETWORKS 64
4.1 Introduction 64
4.2 Results 67
4.3 Discussion 92
4.4 Materials and methods 96
5. A MAGNETICALLY DRIVEN NEUROSPHEROID MICROROBOT FOR TARGETED NEURAL CONNECTION 106
5.1 Introduction 106
5.2 Results and discussion 108
5.3 Materials and methods 116
5.4 Conclusion 122
6. CONCLUSIONS 123
6.1 Overall conclusions 123
6.2 Future work 125
6.2.1 Surface engineering with nanostructures and microprobes 125
6.2.2 In vitro neuronal connections using diverse approaches 126
6.2.3 Improvement in neuronal connections ex vivo 127
6.2.4 In vivo application of the magnetic neurospheroid 128
ACKNOWLEDGEMENTS 129
Publication List 132
REFERENCES 135
요 약 문 147
APPENDIX 149
1. Immunocytochemistry (ICC) protocol combined with tissue clearing 149
2. Confocal imaging using LSM 800 151
3. Electrophysiological analysis using Maxwell Biosystems MaxOne 154

URI

http://dgist.dcollection.net/common/orgView/200000363961
http://hdl.handle.net/20.500.11750/16684

DOI

10.22677/thesis.200000363961

Degree

Doctor

Department

Robotics Engineering

Publisher

DGIST

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