Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Jung Sook | - |
dc.contributor.author | Park, Yu Shin | - |
dc.contributor.author | Nam, Hong Gil | - |
dc.contributor.author | Park, Joon Won | - |
dc.date.available | 2017-05-11T01:52:36Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/1670 | - |
dc.description.abstract | Intracellular localization of RNA is important in regulating various cellular functions. Here, we determined the intracellular localization of the ammonium transporter (AtAMT1;4) mRNA in the sperm cells of Arabidopsis pollen by atomic force microscopy (AFM). The AFM probe was modified with cone-shaped dendron molecules, and an oligonucleotide complementary to the mRNA was attached to the apex. This configuration allowed facile detection of the mRNA within a sectioned pollen cell at the single-molecule level by measuring the mRNA-oligonucleotide interaction force. The AtAMT1;4 mRNA was largely localized in the nucleus rather than the cytosol of the sperm cells. © The Royal Society of Chemistry. 2015. | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Imaging a specific mRNA in pollen with atomic force microscopy | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/c5ra00199d | - |
dc.identifier.scopusid | 2-s2.0-84923361580 | - |
dc.identifier.bibliographicCitation | RSC Advances, v.5, no.24, pp.18858 - 18865 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | LEVEL | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.citation.endPage | 18865 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 18858 | - |
dc.citation.title | RSC Advances | - |
dc.citation.volume | 5 | - |