Detail View

Structural characterization of mouse BEST1 channel
Citations

WEB OF SCIENCE

Citations

SCOPUS

Metadata Downloads

DC Field Value Language
dc.contributor.advisor 김규형 -
dc.contributor.author Ara Ko -
dc.date.accessioned 2022-08-17T16:00:11Z -
dc.date.available 2022-08-17T16:00:11Z -
dc.date.issued 2022 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200000629353 en_US
dc.identifier.uri http://hdl.handle.net/20.500.11750/16791 -
dc.description Bestrophin-1, Calcium-activated chloride channel, Cryo-EM -
dc.description.abstract -
dc.description.statementofresponsibility N -
dc.description.tableofcontents Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 5
2.1 Plasmid construct 5
2.2 Cell culture and transfection 5
2.3 Western Blot analysis 6
2.4 Protein expression and purification in mammalian cell 6
2.5 Negative stain TEM 7
2.6 Cryo-EM data collection and image processing 8
2.7 Protein expression and purification in bacterial cells 9
Ⅲ. RESULTS 14
3.1 Detergent screening and protein purification of mBEST1 14
3.2 Cryo-EM structures of mBEST1 17
3.3 Neck and Ca2+-clasp of mBEST1 and cBEST1 21
3.4 The aperture region of mBEST1 25
3.5 Cl- binding in mBEST1 26
3.6 The C-terminus of mBEST1 27
Ⅳ. DISCUSSION 32
Ⅴ. REFERENCES 35
Ⅵ. Abstract in Korea (요약문) 39
-
dc.format.extent 39 -
dc.language eng -
dc.publisher DGIST -
dc.subject Bestrophin-1, Calcium-activated chloride channel, Cryo-EM -
dc.title Structural characterization of mouse BEST1 channel -
dc.type Thesis -
dc.identifier.doi 10.22677/thesis.200000629353 -
dc.description.degree Master -
dc.contributor.department Department of Brain Sciences -
dc.identifier.bibliographicCitation Ara Ko. (2022). Structural characterization of mouse BEST1 channel. doi: 10.22677/thesis.200000629353 -
dc.contributor.coadvisor Hyun-Ho Lim -
dc.date.awarded 2022/08 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.BM 고62 202208 -
dc.date.accepted 8/11/22 -
dc.contributor.alternativeDepartment 뇌과학과 -
dc.embargo.liftdate 2024-08-31 -
dc.contributor.affiliatedAuthor Ara Ko -
dc.contributor.affiliatedAuthor Kyuhyung Kim -
dc.contributor.affiliatedAuthor Hyun-Ho Lim -
dc.contributor.alternativeName 고아라 -
dc.contributor.alternativeName Kyuhyung Kim -
dc.contributor.alternativeName 임현호 -
Show Simple Item Record

File Downloads

  • There are no files associated with this item.

공유

qrcode
공유하기

Total Views & Downloads