DGIST Scholar: Selective Fluorescent Detection of RNA in Living Cells by Using Imidazolium-Based Cyclophane

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Selective Fluorescent Detection of RNA in Living Cells by Using Imidazolium-Based Cyclophane

Title: Selective Fluorescent Detection of RNA in Living Cells by Using Imidazolium-Based Cyclophane

Author(s): Shirinfar, Bahareh ; Ahmed, Nisar ; Park, Yu Shin ; Cho, Gun-Sik ; Youn, Il Seung ; Han, Jin-Kwan ; Nam, Hong Gil ; Kim, Kwang S.

Subject: Animal Cell ; Animal Model ; Animals ; Aqueous Solution ; Aromatic Moieties ; Caenorhabditis Elegans ; Chelation ; Chemical Structure ; Chemosensor ; Controlled Study ; Cyclophane Derivative ; Cyclophanes ; Cytosol ; Fluorescence ; Fluorescence Enhancement ; Fluorescence Image ; Fluorescent Detection ; Fluorescent Dyes ; H-Bonding ; Hela Cell ; Hela Cells ; Humans ; Hydrogen ; Hydrogen Bond ; Imidazole Derivative ; Imidazoles ; Living Cell ; Macrocyclic Compounds ; Naphthalene ; Non-Human ; Onions ; pH ; Phosphate ; Physiological pH ; Physiology ; Proton ; Proton Nuclear Magnetic Resonance ; RNA ; RNA Binding ; RNA Degradation ; Static Electricity ; Stoichiometry ; Synthesis

Abstract: A water-soluble imidazolium-based fluorescent chemosensor senses RNA selectively through fluorescence enhancement over other biologically relevant biomolecules in aqueous solution at physiological pH 7.4. Fluorescence image detection of RNA in living cells such as onion cells, HeLa cells, and animal model cells was successfully demonstrated which displays a chelation-enhanced fluorescence effect. These affinities can be attributed to the strong electrostatic (C-H)+⋯A- ionic H-bonding and the aromatic moiety driven π-stacking of imidazolium-based cyclophane with the size-complementary major groove of RNA. © 2012 American Chemical Society.