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Polarization-Controlled Photoswitching Resolves Dipole Directions with Subwavelength Resolution
- Polarization-Controlled Photoswitching Resolves Dipole Directions with Subwavelength Resolution
- Lee, Seongsil; Oh, Jungsic; Kim, Dohyeon; Kim, Samuel; Lee, Jong-Bong; Nam, Hong Gil
- DGIST Authors
- Nam, Hong Gil
- Issue Date
- Physical Review Letters, 109(24)
- Article Type
- Biomolecules; Diffraction; Diffraction Limits; Directional Information; DNA Holliday Junction; Fluorescence; Fluorescence Emission; Fluorescent Molecules; Holliday Junctions; Imaging Process; Imaging Techniques; Length Scale; Linearly Polarized; Photoswitching; Polarization; Polarization-Modulated; Sub-Wavelength Resolution
- We present that modulation of fluorescence emission by linearly polarized excitation light can allow us to resolve spatially two fluorescent molecules within a diffraction limit and to determine simultaneously their precise dipole directions. Using polarization-dependent photoswitching, we imaged the 2D geometry of the DNA Holliday junction in a 10-nm length scale by measuring both the distance and the in-plane dipole angle between Cy3 emitters stacked onto the ends of two adjacent branches of the Holliday junction. The proposed polarization-modulated imaging technique provides a simple and nonstochastic imaging process to visualize the nanostructure, including directional information, of biomolecules beyond the diffraction limit. © 2012 American Physical Society.
- AMER PHYSICAL SOC
- Related Researcher
Nam, Hong Gil
CBRG(Complex Biology Research Group)
Plant Aging and Life History; Systems Biology; Complexbiology; Comparative Aging Research
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- Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles
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