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Polarization-Controlled Photoswitching Resolves Dipole Directions with Subwavelength Resolution

Title
Polarization-Controlled Photoswitching Resolves Dipole Directions with Subwavelength Resolution
Authors
Lee, SeongsilOh, JungsicKim, DohyeonKim, SamuelLee, Jong-BongNam, Hong Gil
DGIST Authors
Nam, Hong Gil
Issue Date
2012-12
Citation
Physical Review Letters, 109(24)
Type
Article
Article Type
Article
Keywords
BiomoleculesDiffractionDiffraction LimitsDirectional InformationDNA Holliday JunctionFluorescenceFluorescence EmissionFluorescent MoleculesHolliday JunctionsImaging ProcessImaging TechniquesLength ScaleLinearly PolarizedPhotoswitchingPolarizationPolarization-ModulatedSub-Wavelength Resolution
ISSN
0031-9007
Abstract
We present that modulation of fluorescence emission by linearly polarized excitation light can allow us to resolve spatially two fluorescent molecules within a diffraction limit and to determine simultaneously their precise dipole directions. Using polarization-dependent photoswitching, we imaged the 2D geometry of the DNA Holliday junction in a 10-nm length scale by measuring both the distance and the in-plane dipole angle between Cy3 emitters stacked onto the ends of two adjacent branches of the Holliday junction. The proposed polarization-modulated imaging technique provides a simple and nonstochastic imaging process to visualize the nanostructure, including directional information, of biomolecules beyond the diffraction limit. © 2012 American Physical Society.
URI
http://hdl.handle.net/20.500.11750/1701
DOI
10.1103/PhysRevLett.109.248101
Publisher
AMER PHYSICAL SOC
Related Researcher
  • Author Nam, Hong Gil CBRG(Complex Biology Research Group)
  • Research Interests Plant Aging and Life History; Systems Biology; Complexbiology; Comparative Aging Research
Files:
There are no files associated with this item.
Collection:
Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles


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