Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Finkelstein, Ilya | - |
dc.contributor.author | Zhang, Hongshan | - |
dc.contributor.author | Shi, Zhubing | - |
dc.contributor.author | Kim, Yoori | - |
dc.contributor.author | Yu, Hongtao | - |
dc.contributor.author | Bai, Xiao-Chen | - |
dc.date.accessioned | 2022-12-07T10:40:12Z | - |
dc.date.available | 2022-12-07T10:40:12Z | - |
dc.date.created | 2022-12-07 | - |
dc.date.issued | 2022-04-03 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/17231 | - |
dc.description.abstract | Cohesin and CCCTC-binding factor (CTCF) are key regulatory proteins of three-dimensional (3D) genome organization. I will present direct evidence that cohesin extrudes DNA loops that are anchored by CTCF proteins in specific orientations. CTCF binding polarity controls cohesin-mediated DNA looping. Using single-molecule imaging of CTCF-cohesin collisions, we demonstrate that the N-terminus of CTCF must be oriented towards cohesin to block its ability to compact DNA. C-terminally oriented CTCF accelerates DNA compaction by cohesin. Oriented inactive Cas9 and Cas12a ribonucleoproteins exhibit similar polar effects on DNA compaction by cohesin. RNA-DNA hybrids (R-loops), such as those that occur at highly transcribed genes, efficiently block cohesin-mediated DNA compaction. Our results explain long-standing puzzles regarding how CTCF and transcription shape the 3D genome. © FASEB. | - |
dc.language | English | - |
dc.publisher | Federation of American Societies for Experimental Biology | - |
dc.title | How does cohesin organize the 3D genome? | - |
dc.type | Conference Paper | - |
dc.identifier.doi | 10.1096/fasebj.2022.36.S1.0I131 | - |
dc.identifier.bibliographicCitation | Experimental Biology 2022 | - |
dc.identifier.url | https://www.eventscribe.net/2022/EB2022/biography.asp?pfp=Speakers | - |
dc.citation.conferencePlace | US | - |
dc.citation.conferencePlace | Philadelphia | - |
dc.citation.title | Experimental Biology 2022 | - |
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