Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Hyun, Jung Ho | - |
dc.contributor.author | Nagahama, Kenichiro | - |
dc.contributor.author | Namkung, Ho | - |
dc.contributor.author | Mignocchi, Neymi | - |
dc.contributor.author | Roh, Seung-Eon | - |
dc.contributor.author | Hannan, Patrick | - |
dc.contributor.author | Krüssel, Sarah | - |
dc.contributor.author | Kwak, Chuljung | - |
dc.contributor.author | McElroy, Abigail | - |
dc.contributor.author | Liu, Bian | - |
dc.contributor.author | Cui, Mingguang | - |
dc.contributor.author | Lee, Seunghwan | - |
dc.contributor.author | Lee, Dongmin | - |
dc.contributor.author | Huganir, Richard L. | - |
dc.contributor.author | Worley, Paul F. | - |
dc.contributor.author | Sawa, Akira | - |
dc.contributor.author | Kwon, Hyung-Bae | - |
dc.date.accessioned | 2022-12-15T15:40:10Z | - |
dc.date.available | 2022-12-15T15:40:10Z | - |
dc.date.created | 2022-12-15 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/17238 | - |
dc.description.abstract | Verifying causal effects of neural circuits is essential for proving a direct circuit-behavior relationship. However, techniques for tagging only active neurons with high spatiotemporal precision remain at the beginning stages. Here we develop the soma-targeted Cal-Light (ST-Cal-Light) which selectively converts somatic calcium rise triggered by action potentials into gene expression. Such modification simultaneously increases the signal-to-noise ratio of reporter gene expression and reduces the light requirement for successful labeling. Because of the enhanced efficacy, the ST-Cal-Light enables the tagging of functionally engaged neurons in various forms of behaviors, including context-dependent fear conditioning, lever-pressing choice behavior, and social interaction behaviors. We also target kainic acid-sensitive neuronal populations in the hippocampus which subsequently suppress seizure symptoms, suggesting ST-Cal-Light’s applicability in controlling disease-related neurons. Furthermore, the generation of a conditional ST-Cal-Light knock-in mouse provides an opportunity to tag active neurons in a region- or cell-type specific manner via crossing with other Cre-driver lines. Thus, the versatile ST-Cal-Light system links somatic action potentials to behaviors with high temporal precision, and ultimately allows functional circuit dissection at a single cell resolution. © 2022, The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Tagging active neurons by soma-targeted Cal-Light | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41467-022-35406-y | - |
dc.identifier.scopusid | 2-s2.0-85143994167 | - |
dc.identifier.bibliographicCitation | Nature Communications, v.13, no.1, pp.7692 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | PROJECTION | - |
dc.subject.keywordPlus | SEIZURE | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 7692 | - |
dc.citation.title | Nature Communications | - |
dc.citation.volume | 13 | - |
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