Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Heo, Woojung | - |
dc.contributor.author | Hwang, Hyeonjeong | - |
dc.contributor.author | Kim, Jimin | - |
dc.contributor.author | Oh, Seung Hee | - |
dc.contributor.author | Yu, Youngseok | - |
dc.contributor.author | Lee, Jae-Hyung | - |
dc.contributor.author | Kim, Kyuhyung | - |
dc.date.accessioned | 2023-02-06T09:10:16Z | - |
dc.date.available | 2023-02-06T09:10:16Z | - |
dc.date.created | 2023-02-03 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/17542 | - |
dc.description.abstract | Neuronal differentiation is highly coordinated through a cascade of gene expression, mediated via interactions between trans-acting transcription factors and cis-regulatory elements of their target genes. However, the mechanisms of transcriptional regulation that determine neuronal cell-fate are not fully understood. Here, we show that the nuclear transcription factor Y (NF-Y) subunit, NFYA-1, is necessary and sufficient to express the flp-3 neuropeptide gene in the IL1 neurons of C. elegans. flp-3 expression is decreased in dorsal and lateral, but not ventral IL1s of nfya-1 mutants. The expression of another terminally differentiated gene, eat-4 vesicular glutamate transporter, is abolished, whereas the unc-8 DEG/ENaC gene and pan-neuronal genes are expressed normally in IL1s of nfya-1 mutants. nfya-1 is expressed in and acts in IL1s to regulate flp-3 and eat-4 expression. Ectopic expression of NFYA-1 drives the expression of flp-3 gene in other cell-types. Promoter analysis of IL1-expressed genes results in the identification of several cis-regulatory motifs which are necessary for IL1 expression, including a putative CCAAT-box located in the flp-3 promoter that NFYA-1 directly interacts with. NFYA-1 and NFYA-2, together with NFYB-1 and NFYC-1, exhibit partly or fully redundant roles in the regulation of flp-3 or unc-8 expression, respectively. Taken together, our data indicate that the NF-Y complex regulates neuronal subtype-specification via regulating a set of terminal-differentiation genes. © Korean Society for Biochemistry and Molecular Biology. | - |
dc.language | English | - |
dc.publisher | Korean Society for Biochemistry and Molecular Biology | - |
dc.title | The CCAAT-box transcription factor, NF-Y complex, mediates the specification of the IL1 neurons in C. elegans | - |
dc.type | Article | - |
dc.identifier.doi | 10.5483/BMBRep.2022-0146 | - |
dc.identifier.scopusid | 2-s2.0-85151044642 | - |
dc.identifier.bibliographicCitation | BMB Reports, v.56, no.3, pp.153 - 159 | - |
dc.identifier.kciid | ART002943799 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordAuthor | C. elegans | - |
dc.subject.keywordAuthor | IL1 | - |
dc.subject.keywordAuthor | Neuronal specification | - |
dc.subject.keywordAuthor | NF-Y complex | - |
dc.subject.keywordAuthor | nfya-1 | - |
dc.subject.keywordPlus | TERMINAL SELECTORS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | IDENTITY | - |
dc.subject.keywordPlus | FAMILY | - |
dc.citation.endPage | 159 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 153 | - |
dc.citation.title | BMB Reports | - |
dc.citation.volume | 56 | - |
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