Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Acharya, B[Acharya, Bodhraj] | ko |
dc.contributor.author | Chun, SY[Chun, So-Young] | ko |
dc.contributor.author | Kim, SY[Kim, Shin-Yoon] | ko |
dc.contributor.author | Moon, C[Moon, Cheil] | ko |
dc.contributor.author | Shin, HI[Shin, Hong-In] | ko |
dc.contributor.author | Park, EK[Park, Eui Kyun] | ko |
dc.date.available | 2017-07-05T08:59:26Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.citation | Journal of Biomedical Materials Research Part B: Applied Biomaterials, v.100B, no.3, pp.841 - 849 | - |
dc.identifier.issn | 1552-4973 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/2470 | - |
dc.description.abstract | Calcium phosphate ceramics have been widely used as scaffolds for bone regeneration. Here, to improve the osteogenic potential of hydroxyapatite/ β-tricalcium phosphate (HA/β-TCP) and to apply the bioactive peptide in situ, matrix extracellular phosphoglycoprotein (MEPE) peptide, which has been shown to stimulate osteoblast differentiation, was covalently and directionally immobilized on HA/β-TCP particles. The free-hydroxyl groups on the surface of the HA/β-TCP particles were sequentially conjugated with APTES, PEG-(SS) 2, and the synthetic MEPE peptide. Using FTIR and XPS, immobilization of the MEPE peptide on the HA/β-TCP was confirmed. Implantation of the MEPE peptide-immobilized HA/β-TCP into calvarial defect and subsequent analyses using a micro CT and histology showed significant bone regeneration and increased bone area (9.89-fold) as compared to that of unmodified HA/β-TCP. Moreover, tartrate-resistant acid phosphatase-positive osteoclasts were observed in regenerated bone by the MEPE peptide-immobilized HA/β-TCP, indicating that the bones newly formed by the MEPE peptide-immobilized HA/β-TCP are actively remodeled by osteoclasts. Therefore, our data demonstrate that MEPE peptide immobilization onto the HA/β-TCP surface stimulates bone regeneration associated with physiological bone remodeling. © 2012 WILEY PERIODICALS, INC. | - |
dc.publisher | Wiley Blackwell | - |
dc.subject | Acid Phosphatase Tartrate Resistant Isoenzyme | - |
dc.subject | Animal Experiment | - |
dc.subject | Animal Model | - |
dc.subject | Animals | - |
dc.subject | Bioactive Peptides | - |
dc.subject | Bone | - |
dc.subject | Bone Area | - |
dc.subject | Bone Defect | - |
dc.subject | Bone Mineralization | - |
dc.subject | Bone Regeneration | - |
dc.subject | Bone Remodeling | - |
dc.subject | Calcium Phosphate | - |
dc.subject | Calcium Phosphate Ceramic | - |
dc.subject | Calcium Phosphate Ceramics | - |
dc.subject | Calcium Phosphates | - |
dc.subject | Calvaria | - |
dc.subject | Calvarial Defects | - |
dc.subject | Carboxy Terminal Sequence | - |
dc.subject | Cell Differentiation | - |
dc.subject | Cells, Cultured | - |
dc.subject | Ceramic Particle | - |
dc.subject | Covalent Bond | - |
dc.subject | Disease Models, Animal | - |
dc.subject | Durapatite | - |
dc.subject | Extracellular | - |
dc.subject | Extracellular Matrix Proteins | - |
dc.subject | FT-IR | - |
dc.subject | Glycoproteins | - |
dc.subject | Hematopoietic Stem Cell | - |
dc.subject | Human | - |
dc.subject | Human Cell | - |
dc.subject | Humans | - |
dc.subject | Hydroxyapatite | - |
dc.subject | Immobilized Proteins | - |
dc.subject | In-Situ | - |
dc.subject | Infrared Spectroscopy | - |
dc.subject | Male | - |
dc.subject | Matrix Extracellular Phosphoglycoprotein | - |
dc.subject | MEPE and Bone Marrow Stem Cell | - |
dc.subject | Mice | - |
dc.subject | Mice, Inbred ICR | - |
dc.subject | Micro-Computed Tomography | - |
dc.subject | Micro CT | - |
dc.subject | Mouse | - |
dc.subject | Non-Human | - |
dc.subject | Osteoblast | - |
dc.subject | Osteoblast Differentiation | - |
dc.subject | Osteoblasts | - |
dc.subject | Osteoclast | - |
dc.subject | Osteoclasts | - |
dc.subject | Osteogenic Potential | - |
dc.subject | Peptide Immobilization | - |
dc.subject | Peptides | - |
dc.subject | Phosphatases | - |
dc.subject | Phosphoproteins | - |
dc.subject | Protein Immobilization | - |
dc.subject | Scaffolds (Biology) | - |
dc.subject | Skull Fractures | - |
dc.subject | Stem Cells | - |
dc.subject | Surface Immobilization | - |
dc.subject | Surface Modification | - |
dc.subject | Surface Treatment | - |
dc.subject | Tri-Calcium Phosphates | - |
dc.subject | X Ray Photoelectron Spectroscopy | - |
dc.title | Surface immobilization of MEPE peptide onto HA/ss-TCP ceramic particles enhances bone regeneration and remodeling | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jbm.b.32648 | - |
dc.identifier.wosid | 000300983700029 | - |
dc.identifier.scopusid | 2-s2.0-84862815084 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.contributor.nonIdAuthor | Acharya, B[Acharya, Bodhraj] | - |
dc.contributor.nonIdAuthor | Chun, SY[Chun, So-Young] | - |
dc.contributor.nonIdAuthor | Kim, SY[Kim, Shin-Yoon] | - |
dc.contributor.nonIdAuthor | Shin, HI[Shin, Hong-In] | - |
dc.contributor.nonIdAuthor | Park, EK[Park, Eui Kyun] | - |
dc.identifier.citationVolume | 100B | - |
dc.identifier.citationNumber | 3 | - |
dc.identifier.citationStartPage | 841 | - |
dc.identifier.citationEndPage | 849 | - |
dc.identifier.citationTitle | Journal of Biomedical Materials Research Part B: Applied Biomaterials | - |
dc.type.journalArticle | Article | - |
dc.contributor.affiliatedAuthor | Moon, C[Moon, Cheil] | - |
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