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DC Field Value Language Lee, Eun Soo - Choi, Jung Yoon - Jo, You Hwa - Kim, Joo Yeon - Jang, Yu Jin - Lee, Hye Myeong - Kim, So Yeun - Lee, Ho Jae - Cho, Keun Chang - Jung, Neon Cheol - Hur, Eun Mi - Jeong, Sung Jin - Moon, Cheil - Choe, Youngshik - Rhyu, Im Joo - Kim, Hyun - Sun, Woong - 2017-07-11T04:39:17Z - 2017-04-10 - 2016-01 -
dc.identifier.citation Scientific Reports, v.6 -
dc.identifier.issn 2045-2322 -
dc.identifier.uri -
dc.description.abstract Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues, which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process times. Here, we present the rapid and highly reproducible ACT-PRESTO (active clarity technique-pressure related efficient and stable transfer of macromolecules into organs) method that clears tissues or the whole body within 1 day while preserving tissue architecture and protein-based signals derived from endogenous fluorescent proteins. Moreover, ACT-PRESTO is compatible with conventional immunolabeling methods and expedites antibody penetration into thick specimens by applying pressure. The speed and consistency of this method will allow high-content mapping and analysis of normal and pathological features in intact organs and bodies. -
dc.language English -
dc.publisher Nature Publishing Group -
dc.title ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging -
dc.type Article -
dc.identifier.doi 10.1038/srep18631 -
dc.identifier.wosid 000368127400001 -
dc.identifier.scopusid 2-s2.0-84954117609 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.citation.publicationname Scientific Reports -
dc.contributor.nonIdAuthor Lee, Eun Soo -
dc.contributor.nonIdAuthor Choi, Jung Yoon -
dc.contributor.nonIdAuthor Jo, You Hwa -
dc.contributor.nonIdAuthor Kim, Joo Yeon -
dc.contributor.nonIdAuthor Jang, Yu Jin -
dc.contributor.nonIdAuthor Lee, Hye Myeong -
dc.contributor.nonIdAuthor Kim, So Yeun -
dc.contributor.nonIdAuthor Lee, Ho Jae -
dc.contributor.nonIdAuthor Cho, Keun Chang -
dc.contributor.nonIdAuthor Jung, Neon Cheol -
dc.contributor.nonIdAuthor Hur, Eun Mi -
dc.contributor.nonIdAuthor Jeong, Sung Jin -
dc.contributor.nonIdAuthor Choe, Youngshik -
dc.contributor.nonIdAuthor Rhyu, Im Joo -
dc.contributor.nonIdAuthor Kim, Hyun -
dc.contributor.nonIdAuthor Sun, Woong -
dc.identifier.citationVolume 6 -
dc.identifier.citationTitle Scientific Reports -
dc.type.journalArticle Article -
dc.description.isOpenAccess Y -
dc.subject.keywordPlus AGENT -
dc.subject.keywordPlus MELANIN -
dc.subject.keywordPlus MOUSE-BRAIN -
dc.subject.keywordPlus RECONSTRUCTION -
dc.subject.keywordPlus SINGLE-CELL RESOLUTION -
dc.subject.keywordPlus SYSTemS -
dc.subject.keywordPlus Tomography -
dc.contributor.affiliatedAuthor Lee, Eun Soo -
dc.contributor.affiliatedAuthor Choi, Jung Yoon -
dc.contributor.affiliatedAuthor Jo, You Hwa -
dc.contributor.affiliatedAuthor Kim, Joo Yeon -
dc.contributor.affiliatedAuthor Jang, Yu Jin -
dc.contributor.affiliatedAuthor Lee, Hye Myeong -
dc.contributor.affiliatedAuthor Kim, So Yeun -
dc.contributor.affiliatedAuthor Lee, Ho Jae -
dc.contributor.affiliatedAuthor Cho, Keun Chang -
dc.contributor.affiliatedAuthor Jung, Neon Cheol -
dc.contributor.affiliatedAuthor Hur, Eun Mi -
dc.contributor.affiliatedAuthor Jeong, Sung Jin -
dc.contributor.affiliatedAuthor Moon, Cheil -
dc.contributor.affiliatedAuthor Choe, Youngshik -
dc.contributor.affiliatedAuthor Rhyu, Im Joo -
dc.contributor.affiliatedAuthor Kim, Hyun -
dc.contributor.affiliatedAuthor Sun, Woong -
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Department of Brain Sciences Laboratory of Chemical Senses 1. Journal Articles


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