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A Protein Profile of Visceral Adipose Tissues Linked to Early Pathogenesis of Type 2 Diabetes Mellitus

A Protein Profile of Visceral Adipose Tissues Linked to Early Pathogenesis of Type 2 Diabetes Mellitus
Kim, SJ[Kim, Su-Jin]Chae, S[Chae, Sehyun]Kim, H[Kim, Hokeun]Mun, DG[Mun, Dong-Gi]Back, S[Back, Seunghoon]Choi, HY[Choi, Hye Yeon]Park, KS[Park, Kyong Soo]Hwang, D[Hwang, Daehee]Choi, SH[Choi, Sung Hee]Lee, SW[Lee, Sang-Won]
DGIST Authors
Kim, SJ[Kim, Su-Jin]
Issue Date
Molecular and Cellular Proteomics, 13(3), 811-822
Article Type
Acadl ProteinCalgranulin AChromatography, LiquidClinical ArticleComplement Component C1qComplement Component C1qaControlled StudyDatabases, ProteinDiabetes Mellitus, Type 2Down-RegulationFatty Acid Binding Protein 4Fatty Acid OxidationGlucoseGlucose MetabolismGlucose ToleranceHumanHuman TissueHumansInflammationIntra-Abdominal FatLiquid ChromatographyMass SpectrometryModels, BiologicalMolecular WeightNon-Insulin Dependent Diabetes MellitusOral Glucose Tolerance TestOxidative PhosphorylationPathogenesisPeroxisome Proliferator Activated ReceptorPLIN4 ProteinPriority JournalProteinProtein AnalysisProteomeProteomicsReproducibility of ResultsSignal TransductionSORBS1 ProteinTandem Mass SpectrometryUnclassified DrugUpregulationWestern Blotting
Adipose tissue is increasingly recognized as an endocrine organ playing important pathophysiological roles in metabolic abnormalities, such as obesity, cardiovascular disease, and type 2 diabetes mellitus (T2DM). In particular, visceral adipose tissue (VAT), as opposed to subcutaneous adipose tissue, is closely linked to the pathogenesis of insulin resistance and T2DM. Despite the importance of VAT, its molecular signatures related to the pathogenesis of T2DM have not been systematically explored. Here, we present comprehensive proteomic analysis of VATs in drug-naïve early T2DM patients and subjects with normal glucose tolerance. A total of 4,707 proteins were identified in LC-MS/MS experiments. Among them, 444 increased in abundance in T2DM and 328 decreased. They are involved in T2DM-related processes including inflammatory responses, peroxisome proliferator-activated receptor signaling, oxidative phosphorylation, fatty acid oxidation, and glucose metabolism. Of these proteins, we selected 11 VAT proteins that can represent alteration in early T2DM patients. Among them, up-regulation of FABP4, C1QA, S100A8, and SORBS1 and down-regulation of ACADL and PLIN4 were confirmed in VAT samples of independent early T2DM patients using Western blot. In summary, our profiling provided a comprehensive basis for understanding the link of a protein profile of VAT to early pathogenesis of T2DM. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
American Society for Biochemistry and Molecular Biology Inc.
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