Cited 4 time in webofscience Cited 7 time in scopus

Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound

Title
Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound
Authors
Yoon, SangpilKim, Min GonChiu, Chi TatHwang, Jae YounKim, Hyung HamWang, YingxiaoShung, K. Kirk
DGIST Authors
Hwang, Jae Youn
Issue Date
2016-02
Citation
Scientific Reports, 6
Type
Article
Article Type
Article
ISSN
2045-2322
Abstract
Controlling cell functions for research and therapeutic purposes may open new strategies for the treatment of many diseases. An efficient and safe introduction of membrane impermeable molecules into target cells will provide versatile means to modulate cell fate. We introduce a new transfection technique that utilizes high frequency ultrasound without any contrast agents such as microbubbles, bringing a single-cell level targeting and size-dependent intracellular delivery of macromolecules. The transfection apparatus consists of an ultrasonic transducer with the center frequency of over 150 MHz and an epi-fluorescence microscope, entitled acoustic-transfection system. Acoustic pulses, emitted from an ultrasonic transducer, perturb the lipid bilayer of the cell membrane of a targeted single-cell to induce intracellular delivery of exogenous molecules. Simultaneous live cell imaging using HeLa cells to investigate the intracellular concentration of Ca2+ and propidium iodide (PI) and the delivery of 3 kDa dextran labeled with Alexa 488 were demonstrated. Cytosolic delivery of 3 kDa dextran induced via acoustic-transfection was manifested by diffused fluorescence throughout whole cells. Short-term (6 hr) cell viability test and long-term (40 hr) cell tracking confirmed that the proposed approach has low cell cytotoxicity.
URI
http://hdl.handle.net/20.500.11750/2725
DOI
10.1038/srep20477
Publisher
Nature Publishing Group
Related Researcher
  • Author Hwang, Jae Youn MBIS(Multimodal Biomedical Imaging and System) Laboratory
  • Research Interests Multimodal Imaging; High-Frequency Ultrasound Microbeam; Ultrasound Imaging and Analysis; 스마트 헬스케어
Files:
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Collection:
Department of Information and Communication EngineeringMBIS(Multimodal Biomedical Imaging and System) Laboratory1. Journal Articles


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