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NON-CONTACT HIGH-FREQUENCY ULTRASOUND MICROBEAM STIMULATION FOR STUDYING MECHANOTRANSDUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
- NON-CONTACT HIGH-FREQUENCY ULTRASOUND MICROBEAM STIMULATION FOR STUDYING MECHANOTRANSDUCTION IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
- Hwang, JY[Hwang, Jae Youn]; Lim, HG[Lim, Hae Gyun]; Yoon, CW[Yoon, Chi Woo]; Lam, KH[Lam, Kwok Ho]; Yoon, S[Yoon, Sangpil]; Lee, C[Lee, Changyang]; Chiu, CT[Chiu, Chi Tat]; Kang, BJ[Kang, Bong Jin]; Kim, HH[Kim, Hyung Ham]; Shung, KK[Shung, K. Kirk]
- DGIST Authors
- Hwang, JY[Hwang, Jae Youn]
- Issue Date
- Ultrasound in Medicine and Biology, 40(9), 2172-2182
- Article Type
- Acoustic Pressures; Acoustic Stimulation; Auditory Stimulation; Calcium; Calcium Cell Level; Calcium Fluorescence Imaging; Calcium Signaling; Cell Stimulation; Cellular Response; Controlled Study; Devices; Echography; Endothelial Cells; Fluorescence Imaging; Fluorescence Microscopy; High-Energy Shock Waves; High-Frequency Ultrasound Microbeam; High Frequency Ultrasound Microbeam Stimulation; High Frequency Ultrasounds; Highly Focused Ultrasounds; Human; Human Cell; Human Umbilical Vein Endothelial Cells; Humans; Mechanical Stimulation; Mechanotransduction; Mechanotransduction, Cellular; Metabolism; Microscopy, Fluorescence; Optical Imaging; Physiology; Priority Journal; Procedures; Procedures Concerning Cells; Radiation; Sound Intensity; Ultrasonics; Ultrasound; Umbilical Vein Endothelial Cell
- We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified. © 2014 World Federation for Ultrasound in Medicine & Biology.
- Related Researcher
Hwang, Jae Youn
MBIS(Multimodal Biomedical Imaging and System) Laboratory
Multimodal Imaging; High-Frequency Ultrasound Microbeam; Ultrasound Imaging and Analysis; 스마트 헬스케어
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- Department of Information and Communication EngineeringMBIS(Multimodal Biomedical Imaging and System) Laboratory1. Journal Articles
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