Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Choi, Seong-Kyoon | - |
dc.contributor.author | Park, Jin-Kyu | - |
dc.contributor.author | Lee, Kyeong-Min | - |
dc.contributor.author | Lee, Soo-Keun | - |
dc.contributor.author | Jeon, Won Bae | - |
dc.date.available | 2017-07-11T06:32:33Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2013-11 | - |
dc.identifier.issn | 1552-4973 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/3188 | - |
dc.description.abstract | Poly(lactide-co-glycolide) (PLGA) and elastin-like polypeptide (ELP) have been widely used as a biodegradable scaffold and thermoresponsive matrix, respectively. However, little attention has focused on the combinatorial use of these biomaterials for tissue engineering applications. An ELP matrix TGPG[VGRGD(VGVPG)6]20WPC (referred to as REP) contains multiple Arg-Gly-Asp motifs. This study fabricated porous PLGA scaffolds coated with various concentration of matrix via thermally induced phase transition to improve adhesion-mediated proliferation and differentiation of neural progenitor cells. Matrix-coated scaffolds were characterized by FTIR, SEM, and hematoxylin and eosin staining with respect to coating efficiency, porosity, and pore size and shape. On the matrix-coated scaffolds, cells grew as a single cell or associated each other to form a multicellular layer or cluster. In biological evaluations, cell adhesion and proliferation were significantly promoted in a matrix concentration-dependent manner. More importantly, in combination with retinoic acid, the differentiation of progenitor cells into neuronal and astroglial lineages was highly stimulated in the cells cultured on matrix-coated scaffolds than on untreated controls. Taken together, our results indicated that the REP matrix-functionalized PLGA scaffolds are suitable for improving neuronal cell functions, and thus applicable for neural tissue engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1329-1339, 2013. Copyright © 2013 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | Wiley Blackwell | - |
dc.title | Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jbm.b.32950 | - |
dc.identifier.scopusid | 2-s2.0-84886589420 | - |
dc.identifier.bibliographicCitation | Journal of Biomedical Materials Research Part B: Applied Biomaterials, v.101, no.8, pp.1329 - 1339 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | PLGA scaffolds | - |
dc.subject.keywordAuthor | extracellular matrix | - |
dc.subject.keywordAuthor | surface modification | - |
dc.subject.keywordAuthor | stem | - |
dc.subject.keywordAuthor | progenitor cells | - |
dc.subject.keywordAuthor | tissue engineering | - |
dc.subject.keywordPlus | PERIPHERAL-NERVE REGENERATION | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | SURFACE MODIFICATION | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | CHITOSAN | - |
dc.subject.keywordPlus | FIBRONECTIN | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.citation.endPage | 1339 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1329 | - |
dc.citation.title | Journal of Biomedical Materials Research Part B: Applied Biomaterials | - |
dc.citation.volume | 101 | - |
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