Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyeong-Min | - |
dc.contributor.author | Jung, Gwon-Soo | - |
dc.contributor.author | Park, Jin-Kyu | - |
dc.contributor.author | Choi, Seong-Kyoon | - |
dc.contributor.author | Jeon, Won Bae | - |
dc.date.available | 2017-07-11T06:37:26Z | - |
dc.date.created | 2017-04-10 | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 1742-7061 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/3259 | - |
dc.description.abstract | Extracellular matrix (ECM) plays an important role in controlling the β-cell morphology, survival and insulin secretary functions. An RGD-modified elastin-like polypeptide (RGD-ELP), TGPG[VGRGD(VGVPG) 6]20WPC, has been reported previously as a bioactive matrix. In this study, to investigate whether RGD-ELP affects β-cell growth characteristics and insulin secretion, β-TC6 cells were cultured on the RGD-ELP coatings prepared via thermally induced phase transition. On RGD-ELP, β-TC6 cells clustered into an islet-like architecture with high cell viability. Throughout 7 days' culture, the proliferation rate of the cells within a pseudoislet was similar to that of monolayer culture. Under high glucose (25 mM), β-TC6 pseudoislets showed up-regulated insulin gene expression and exhibited glucose-stimulated insulin secretion. Importantly, the mRNA and protein abundances of cell adhesion molecules (CAM) E-cadherin and connexin-36 were much higher in pseudoislets than in monolayer cells. The siRNA-mediated inhibition of E-cadherin or connexin-36 expression severely limited pseudoislet formation. In addition, the mRNA levels of collagen types I and IV, fibronectin and laminin were significantly elevated in pseudoislets. The results suggest that RGD-ELP promotes pseudoislet formation via up-regulation of the CAM and ECM components. The functional roles of RGD-ELP are discussed in respect of its molecular composition. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Effects of Arg-Gly-Asp-modified elastin-like polypeptide on pseudoislet formation via up-regulation of cell adhesion molecules and extracellular matrix proteins | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.actbio.2012.10.036 | - |
dc.identifier.scopusid | 2-s2.0-84873184802 | - |
dc.identifier.bibliographicCitation | Acta Biomaterialia, v.9, no.3, pp.5600 - 5608 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | Beta-cells | - |
dc.subject.keywordAuthor | Pseudoislet | - |
dc.subject.keywordAuthor | Insulin secretion | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.subject.keywordAuthor | Connexin-36 | - |
dc.subject.keywordPlus | PANCREATIC BETA-CELLS | - |
dc.subject.keywordPlus | INSULIN-SECRETION | - |
dc.subject.keywordPlus | ISLET TRANSPLANTATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | CX36 | - |
dc.subject.keywordPlus | CA2+ | - |
dc.subject.keywordPlus | RGD | - |
dc.citation.endPage | 5608 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 5600 | - |
dc.citation.title | Acta Biomaterialia | - |
dc.citation.volume | 9 | - |
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