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C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface
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Title
C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface
Issued Date
2011-11
Citation
Chemical Communications, v.47, no.43, pp.11900 - 11902
Type
Article
Keywords
3(4) Vinylbenzyl 12 Phosphocholine Dodecanoate ; Adsorption ; Antibodies ; Antibodies, Immobilized ; Article ; Binding ; Binding Affinity ; C-Reactive Protein ; C Reactive Protein ; Human ; Humans ; Immunoassay ; Ligand ; Ligands ; Molecular Imprinting ; MOLECULARLY IMPRINTED POLYMERS ; Nonhuman ; Oils ; Phosphorylcholine ; Polymer ; Polymerization ; Polystyrenes ; Protein Analysis ; Protein Immobilization ; RECOGNITION ; SERUM ; Solid ; Styrene ; SUPPORTED LIPID MONOLAYERS ; Surface Properties ; Unclassified Drug ; WATER
ISSN
1359-7345
Abstract
The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated. © 2011 The Royal Society of Chemistry.
URI
http://hdl.handle.net/20.500.11750/3491
DOI
10.1039/c1cc15079k
Publisher
Royal Society of Chemistry
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