DGIST Scholar: Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance

Division of Biotechnology 1. Journal Articles

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Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance

Title: Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance

Author(s): Kim, Hyun Chul ; Lee, Soo Keun ; Jeon, Won Bae ; Lyu, Hong-Kun ; Lee, Seung Woo ; Jeong, Sang Won

DGIST Authors: Kim, Hyun Chul ; Lee, Soo Keun ; Jeon, Won Bae ; Lyu, Hong-Kun ; Lee, Seung Woo ; Jeong, Sang Won

Author Keywords: Poly(thiophene) ; Self-assembled monolayer ; Surface plasmon resonance ; Biosensor

Keywords: Self Assembly ; Sensors ; Surface Plasmon Resonance ; Surface Plasmon Resonance ; Surface Plasmon Resonance ; Thiophene ; Organic Compounds ; Organic Polymers ; Ester Derivative ; Ester Groups ; Esterification ; Esters ; Ferric Chloride ; Poly(3 (2 ((N Succinimidyl)Succinyloxy)Ethyl)Thiophene) ; Poly(Thiophene) ; Poly(Thiophene) ; Polymer ; Polymerization ; Polymers ; Protein Analysis ; Protein Interaction ; RISK ; Self-Assembled Monolayer ; Self-Assembled Monolayer ; Thiophene Derivative ; Thiophenes ; Gel Permeation Chromatography ; Unclassified Drug ; ACUTE-PHASE RESPONSE ; Amine ; Antibody ; Article ; Biosensing Techniques ; Biosensor ; Biosensor ; C-Reactive Protein ; C-Reactive Protein ; C Reactive Protein ; C Reactive Protein Antibody ; Chloroform ; DISEASE ; ELECTRICAL DETECTION ; Electrodes ; GOLD ; GOLD ; Immobilization ; IMMUNOSENSOR ; Inflammation ; Infrared Spectroscopy ; LABEL-FREE ; Molecular Biology ; N-Hydroxysuccinimide ; N Hydroxysuccinimide ; Nuclear Magnetic Resonance

Abstract: The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system. © 2008 Elsevier B.V. All rights reserved.