Multimodal Quantitative Microscopic System based on Ultrasound and Optical Technique
- Title
- Multimodal Quantitative Microscopic System based on Ultrasound and Optical Technique
- Alternative Title
- 초음파 및 광학 기술 기반 정량적 다중모달 현미경 시스템 개발
- Author(s)
- Sangyeon Youn
- DGIST Authors
- Sangyeon Youn ; Jae Youn Hwang ; Kijoon Lee
- Advisor
- 황재윤
- Co-Advisor(s)
- Kijoon Lee
- Issued Date
- 2023
- Awarded Date
- 2023-02-01
- Type
- Thesis
- Description
- Multimodal Imaging system and Analysis, Optical Imaging Technique, Quantitative Phase Imaging, Acoustic tweezer, Fluorescent Imaging System
- Table Of Contents
-
1. INTRODUCTION 1
1.1 Cancer Cell Characterization for Elucidation of Biological Phenomena 1
1.2 Conventional Methods of Estimation of Invasion Potentials 4
1.3 Academic Significance and Hypothesis 7
1.4 Overview of Thesis 10
2. THEORETICAL BACKGROUND 12
2.1 Ultrasound Transducer 12
2.1.1 Piezoelectric Materials 13
2.1.2 Matching Layer 16
2.1.3 Backing Layer 18
2.1.4 Single-element Ultrasound Transducer 18
2.2 Acoustic Trapping Technique 20
2.2.1 Various Type of Acoustic Trapping 20
2.2.2 Basic Principle of the Acoustic Tweezer Technique 20
2.2.3 Application of Acoustic Tweezer Technique 22
2.2.4 What is Differ from Optical Tweezers 23
2.3 Fluorescence Imaging 25
2.3.1 Basic Principle of the Fluorescence Imaging 25
2.3.2 Fluorescence Imaging and Calcium Ion Imaging in Bioscience 27
2.4 Quantitative Phase Imaging 30
2.4.1 What is Differ from the Bright-field Microscope 30
2.4.2 Basic Principle of Quantitative Phase Imaging 31
2.4.3 Introduction of Full-field QPI and Imaging Sensitivity 32
2.4.4 Strength Points of Quantitative Phase Imaging 34
3. Acoustic Trapping Technique for Studying Calcium Response of a Suspended Breast Cancer Cell: Determination of its Invasion Potentials 36
3.1 Introduction 36
3.2 Materials and Methods 39
3.2.1 Single-beam Acoustic Trapping System 39
3.2.2 Press-focused Single-crystal Ultrasound Transducer 40
3.2.3 Cell Preparation 42
3.2.4 Monitoring of Intracellular Calcium Elevation of Breast Cancer Cells While Acoustic Trapping 43
3.2.5 Cell Viability Test 44
3.3 Results 45
3.3.1 Calcium Response of a Trapped Cell to Acoustic Trapping Force at Different Voltages 45
3.3.2 Quantitative Analysis of Calcium Responses of MDAMB-231 and MCF-7 Cells to Acoustic Trapping Forces 47
3.3.3 Changes in Cell Viability due to Acoustic Trapping 49
3.4 Discussion 52
3.5 Conclusion 54
4. Fully-Automatic Deep Learning-Based Analysis for Determination of Invasiveness of Breast Cancer Cells in an Acoustic Trap 56
4.1 Introduction 56
4.1.1 Motivation 56
4.1.2 Contributions 58
4.1.3 Related Works 59
4.2 Materials and Methods 60
4.2.1 High-frequency Ultrasound Single beam Acoustic Trapping System with Deep learning-based Calcium Image Analysis 60
4.2.2 High-frequency Single Element Focused Ultrasound Transducer 62
4.2.3 Multi-scale and Multi-channel Deep Learning Network (MM-Net) for Segmentation of Fluorescent Cells 63
4.2.4 Automatic Calcium Analysis Algorithm for a Trapped Single Cell in Time-lapse Fluorescence Images 69
4.2.5 Cell Preparation and Experimental Setup 73
4.3 Results 74
4.3.1 Automatic Cell Segmentation 74
4.3.2 Automatic Calcium Analysis of a Trapped Cell in Acoustic Beam 76
4.3.3 Quantitative Analysis of Calcium Responses of the MDA-MB-231 and MCF-7 Cells to Different Acoustic Trapping Forces 79
4.4 Discussion 81
4.5 Conclusion 85
5. Development of a Cell Elastographic Microscope System based on Quantitative Phase Imaging and Acoustic Trapping Techniques for Characterizing Cancer Cells 86
5.1 Introduction 86
5.2 Materials and Methods 90
5.2.1 System Configuration of Cell Elastographic Microscope 90
5.2.2 High-Frequency Ring-shaped Ultrasound Transducer 93
5.2.3 Quantitative Phase Image Reconstruction and Image Processing 94
5.2.4 Experimental Set-up for Quantitative Measurement of Mechanical Properties of a Cell 96
5.2.5 Tissue Mimicking Elastic Phantom Fabrication 97
5.2.6 Cell Preparation 98
5.3 Results 99
5.3.1 Assessment of Optical Performance of the Quantitative Phase Imaging System 99
5.3.2 Measurement of Deformation Rate of Elastic Gelatin Phantoms under Varied Acoustic Pressure 101
5.3.3 Quantitative Comparison of Transverse Deformation Rate of MDA-MB-231 and MCF-7 cells 102
5.3.4 Measurement of Thickness Change in MDA-MB-231and MCF-7 cells during Acoustic Trapping 104
5.3.5 Comparison of Representative Cell Thickness Changes of Both Cells under 1.24 and 3.18MPa 107
5.3.6 Quantitative Analysis of Displacement of MDA-MB-231 and MCF-7 cells due to Acoustic Trapping Force 108
5.4 Discussion 109
5.5 Conclusion 112
6. CONCLUSION 114
BIBLIOGRAPHY 118
- URI
-
http://hdl.handle.net/20.500.11750/45688
http://dgist.dcollection.net/common/orgView/200000655117
- Degree
- Doctor
- Publisher
- DGIST
- Related Researcher
-
-
Hwang, Jae Youn
- Research Interests Multimodal Imaging; High-Frequency Ultrasound Microbeam; Ultrasound Imaging and Analysis; 스마트 헬스케어; Biomedical optical system
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- Appears in Collections:
- Department of Electrical Engineering and Computer Science Theses Ph.D.
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