Title

Electrophysiological characterization of Bestrophin channels in C. elegans

Alternative Title

예쁜꼬마선충 베스트로핀 채널의 전기생리학적 특성

DGIST Authors

Batnasan Sunjidmaa ; Kyuhyung Kim ; Hyun-Ho Lim

Advisor

김규형

Co-Advisor(s)

Hyun-Ho Lim

Issued Date

2023

Awarded Date

2023-02-01

Citation

Batnasan Sunjidmaa. (2023). Electrophysiological characterization of Bestrophin channels in C. elegans. doi: 10.22677/THESIS.200000659237

Type

Thesis

Description

Bestrophin (BEST) channels, Calcium-activated chloride channel, C. elegans, Electrophysiology

Table Of Contents

Introduction 1
MATERIALS AND METHODS 4
2.1 Cell culture and transfection 4
2.3 Western blot analysis 4
2.4 Electrophysiological recordings 4
2.5 Data analysis 4
RESULTS 9
3.1 The phylogenetic analysis of ceBEST channel and sequence alignment of Bestrophin channels 9
3.2 Heterologous expression of ceBEST in HEK293T cell 14
3.3 Electrophysiological characterization C. elegans BEST5 15
3.4 Electrophysiological characterization C. elegans BEST13 17
3.5 Electrophysiological characterization C. elegans BEST14 20
3.6 Electrophysiological characterization C. elegans BEST19 23
DISCUSSION 26
REFERENCES 29
Abstract in Korea (요약문) 32

URI

http://hdl.handle.net/20.500.11750/45694
http://dgist.dcollection.net/common/orgView/200000659237

DOI

10.22677/THESIS.200000659237

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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