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Electrophysiological characterization of Bestrophin channels in C. elegans

Title
Electrophysiological characterization of Bestrophin channels in C. elegans
Alternative Title
예쁜꼬마선충 베스트로핀 채널의 전기생리학적 특성
Author(s)
Batnasan Sunjidmaa
DGIST Authors
Batnasan SunjidmaaKyuhyung KimHyun-Ho Lim
Advisor
김규형
Co-Advisor(s)
Hyun-Ho Lim
Issued Date
2023
Awarded Date
2023-02-01
Type
Thesis
Description
Bestrophin (BEST) channels, Calcium-activated chloride channel, C. elegans, Electrophysiology
Table Of Contents
Introduction 1
MATERIALS AND METHODS 4
2.1 Cell culture and transfection 4
2.3 Western blot analysis 4
2.4 Electrophysiological recordings 4
2.5 Data analysis 4
RESULTS 9
3.1 The phylogenetic analysis of ceBEST channel and sequence alignment of Bestrophin channels 9
3.2 Heterologous expression of ceBEST in HEK293T cell 14
3.3 Electrophysiological characterization C. elegans BEST5 15
3.4 Electrophysiological characterization C. elegans BEST13 17
3.5 Electrophysiological characterization C. elegans BEST14 20
3.6 Electrophysiological characterization C. elegans BEST19 23
DISCUSSION 26
REFERENCES 29
Abstract in Korea (요약문) 32
URI
http://hdl.handle.net/20.500.11750/45694

http://dgist.dcollection.net/common/orgView/200000659237
DOI
10.22677/THESIS.200000659237
Degree
Master
Department
Department of Brain Sciences
Publisher
DGIST
Related Researcher
  • 김규형 Kim, Kyuhyung
  • Research Interests Neurobehavior; Neural Circuit; Neurodevelopment; 신경회로; 신경행동; 신경발생; 신경유전학
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Department of Brain Sciences Theses Master

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