Title

The role of astrocytic phagocytosis in the adult medial prefrontal cortex

Alternative Title

성체 전전두피질에서 성상교세포 매개 식세포작용의 역할

DGIST Authors

Seoyoon Park ; Han Kyoung Choe ; Hyungju Park

Advisor

최한경

Co-Advisor(s)

Hyungju Park

Issued Date

2023

Awarded Date

2023-02-01

Citation

Seoyoon Park. (2023). The role of astrocytic phagocytosis in the adult medial prefrontal cortex. doi: 10.22677/THESIS.200000654721

Type

Thesis

Description

Astrocyte, Phagocytosis, Medial prefrontal cortex, Synapse, Working memory

Table Of Contents

I. INTRODUCTION 1
1.1 Medial prefrontal cortex and its roles in cognitive functions 1
1.2 Astrocytic phagocytosis mechanisms and their roles in synapse refinement 1
1.3 Possible roles of astrocytic phagocytosis in mPFC circuit homeostasis 2
II. MATERIALS AND METHODS 3
2.1 Animals 3
2.2 AAV stereotaxic injection 3
2.3 Whole cell patch clamp 3
2.4 Delayed-Non-Match-to-Place (DNMTP) Task 4
2.5 Immunohistochemistry 5
2.6 Analysis of confocal image 5
2.7 Statistical analysis 5
III. RESULTS 6
3.1 Mouse model for downregulating astrocytic phagocytosis mechanisms in the adult mPFC 6
3.2 Normal spontaneous excitatory synaptic transmission by astrocytic Megf10/Metrk KO 7
3.3 Normal spontaneous inhibitory synaptic transmission by astrocytic Megf10/Metrk KO 9
3.4 Normal excitatory synaptic structures in the mPFC with impaired astrocytic synapse phagocytosis 11
3.5 Normal inhibitory synaptic structures in the mPFC with impaired astrocytic synapse phagocytosis 13
3.6 Role of astrocytic synapse phagocytosis in mPFC-dependent working memory formation 16
IV. DISCUSSION 21
4.1 Possibility of synapse elimination through other mechanisms 21
4.1.1 Another phagocytic receptor-mediated synapse elimination in astrocyte 21
4.1.2 Microglia-mediated synapse elimination 22
4.1.3 Neuronal activity-dependent competition 22
4.2 Possibility of mPFC sub-region or layer-specific manner 23
V REFERENCE 24

URI

http://hdl.handle.net/20.500.11750/45700
http://dgist.dcollection.net/common/orgView/200000654721

DOI

10.22677/THESIS.200000654721

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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