Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김규형 | - |
| dc.contributor.author | Eunbi Kim | - |
| dc.date.accessioned | 2023-03-22T19:56:35Z | - |
| dc.date.available | 2023-03-22T19:56:35Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/45701 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000658331 | - |
| dc.description | TREM2, Alzheimer’s disease, microglia, therapeutic, monoclonal antibodies, hybridoma | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 5 2.1 Expression and Purification of hTREM2174 5 2.2 Proteolytic digestion of hTREM2174 6 2.3 Immunization and hybridoma fusion 6 2.4 ELISA (Enzyme-linked immunosorbent assay) 7 2.5 Purification of TREM2 monoclonal antibody 8 2.6 Sequence determination of TREM2 monoclonal antibodies 8 2.7 Mouse genotyping 10 Ⅲ. RESULTS 12 3.1 Purification of TREM2 ectodomain 12 3.2 Antibody generation against hTREM2 ectodomain 17 3.3 Antibody subtyping and production 21 3.4 Genotyping of the hTREM2 knock-in mouse 27 Ⅳ. DISCUSSION 30 Ⅴ. REFERENCES 33 Ⅵ. Abstract in Korea (요약문) 35 |
- |
| dc.format.extent | 35 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Development of monoclonal antibodies for the functional modulation of human TREM2 signaling | - |
| dc.title.alternative | 인간 TREM2 신호전달의 기능적 조절을 위한 단클론 항체의 개발 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000658331 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | Department of Brain Sciences | - |
| dc.contributor.coadvisor | Hyun-Ho Lim | - |
| dc.date.awarded | 2023-02-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.BM 김67 202302 | - |
| dc.date.accepted | 2023-03-21 | - |
| dc.contributor.alternativeDepartment | 뇌과학과 | - |
| dc.subject.keyword | TREM2 | - |
| dc.subject.keyword | Alzheimer’s disease | - |
| dc.subject.keyword | microglia | - |
| dc.subject.keyword | therapeutic | - |
| dc.subject.keyword | monoclonal antibodies | - |
| dc.subject.keyword | hybridoma | - |
| dc.contributor.affiliatedAuthor | Eunbi Kim | - |
| dc.contributor.affiliatedAuthor | Kyuhyung Kim | - |
| dc.contributor.affiliatedAuthor | Hyun-Ho Lim | - |
| dc.contributor.alternativeName | 김은비 | - |
| dc.contributor.alternativeName | Kyuhyung Kim | - |
| dc.contributor.alternativeName | 임현호 | - |
| dc.rights.embargoReleaseDate | 2025-02-28 | - |
- Files in This Item:
-
There are no files associated with this item.
- Appears in Collections:
- Department of Brain Sciences Theses Master
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.