Title

Enhanced magnetic manipulation of bio-functionalized cells and their membrane elasticity analysis

DGIST Authors

Ali Abbas ; CheolGi Kim ; Chun-Yeol You

Advisor

김철기

Co-Advisor(s)

Chun-Yeol You

Issued Date

2023

Awarded Date

2023-02-01

Citation

Ali Abbas. (2023). Enhanced magnetic manipulation of bio-functionalized cells and their membrane elasticity analysis. doi: 10.22677/THESIS.200000656095

Type

Thesis

Description

Bio-functionalized particles, magnetophoresis, gradient field, Railway track, energy landscape, bio-entities.

Table Of Contents

Ⅰ. Introduction 1
1.1 Magnetic bio-functionalized cells 2
1.2 Significance of magnetic manipulation for bio-functionalized particles 3
1.3 Micromagnetic pattern for the manipulation of bio-functionalized particles 4
1.4 External field directionality and magnetic bead manipulation 5
1.5 Enhanced manipulation of bio-functionalized particles 7
1.6 Cell membrane plasticity/elasticity measurement via magnetic force 10
II. Theoretical Background 12
2.1 Various external forces for the manipulation of bio-functionalized particles 12
2.2 Magnetophoresis 13
2.3 Effective magnetic force acting on bio-functionalized particles 13
2.3.1 Gradient magnetic force 13
2.4 Various methods for the cell mechanical properties analysis 15
III. Materials and Methods 16
3.1 Materials 16
3.2 On-chip micro-patterned device fabrication 16
3.2.1 Substrate cleaning 16
3.2.2 Photolithography 17
3.2.3 Sputtering (magnetic thin film deposition) 18
3.2.4 Electrochemical deposition for disk and half-disk patterns 18
3.2.5 Teflon coating as passivation layer 19
3.3 Simulation of Railway magnetic texture for the fixed directionality 20
3.4 Bio-functionalization of magnetic particles 24
3.5 Cell conjugation with magnetic particles 25
3.5.1 Binding of HLA-A2/EpCAM antibodies to the magnetic particles 25
3.5.2 Cell Culturing and growth 25
3.5.3 Binding of cells and SPM particles 26
3.6 Experimental set-up 26
IV. Results and Discussions 28
4.1 Railway surface texture for enhanced manipulation of magnetic beads 28
4.1.1 Linear Railway-track 28
4.1.2 Classification and optimization of the Railway unit structure 29
4.1.3 Particle movement on the T and S-shaped Railway-track 31
4.1.4 Complex maze shaped Railway-track for the particle manipulation 32
4.1.5 Railway-track as a particle collector 34
4.1.6 Railway-track as an integrated particle isolator 36
4.2 Cell membrane elasticity measurement with the magnetophoretic device 39
Conclusion 43
References 44

URI

http://hdl.handle.net/20.500.11750/45770
http://dgist.dcollection.net/common/orgView/200000656095

DOI

10.22677/THESIS.200000656095

Degree

Master

Department

Department of Physics and Chemistry

Publisher

DGIST

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