Bio-functionalized particles, magnetophoresis, gradient field, Railway track, energy landscape, bio-entities.
Table Of Contents
Ⅰ. Introduction 1 1.1 Magnetic bio-functionalized cells 2 1.2 Significance of magnetic manipulation for bio-functionalized particles 3 1.3 Micromagnetic pattern for the manipulation of bio-functionalized particles 4 1.4 External field directionality and magnetic bead manipulation 5 1.5 Enhanced manipulation of bio-functionalized particles 7 1.6 Cell membrane plasticity/elasticity measurement via magnetic force 10 II. Theoretical Background 12 2.1 Various external forces for the manipulation of bio-functionalized particles 12 2.2 Magnetophoresis 13 2.3 Effective magnetic force acting on bio-functionalized particles 13 2.3.1 Gradient magnetic force 13 2.4 Various methods for the cell mechanical properties analysis 15 III. Materials and Methods 16 3.1 Materials 16 3.2 On-chip micro-patterned device fabrication 16 3.2.1 Substrate cleaning 16 3.2.2 Photolithography 17 3.2.3 Sputtering (magnetic thin film deposition) 18 3.2.4 Electrochemical deposition for disk and half-disk patterns 18 3.2.5 Teflon coating as passivation layer 19 3.3 Simulation of Railway magnetic texture for the fixed directionality 20 3.4 Bio-functionalization of magnetic particles 24 3.5 Cell conjugation with magnetic particles 25 3.5.1 Binding of HLA-A2/EpCAM antibodies to the magnetic particles 25 3.5.2 Cell Culturing and growth 25 3.5.3 Binding of cells and SPM particles 26 3.6 Experimental set-up 26 IV. Results and Discussions 28 4.1 Railway surface texture for enhanced manipulation of magnetic beads 28 4.1.1 Linear Railway-track 28 4.1.2 Classification and optimization of the Railway unit structure 29 4.1.3 Particle movement on the T and S-shaped Railway-track 31 4.1.4 Complex maze shaped Railway-track for the particle manipulation 32 4.1.5 Railway-track as a particle collector 34 4.1.6 Railway-track as an integrated particle isolator 36 4.2 Cell membrane elasticity measurement with the magnetophoretic device 39 Conclusion 43 References 44