WEB OF SCIENCE
SCOPUS
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이성배 | - |
| dc.contributor.author | Eunmi Lee | - |
| dc.date.accessioned | 2023-03-22T19:57:59Z | - |
| dc.date.available | 2023-03-22T19:57:59Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/45780 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000658141 | - |
| dc.description | Alzheimer's disease, Tauopathy, Glutaredoxin 1, Templated aggregation of tau, Reactive oxygen species, Apoptosis, Autophagy | - |
| dc.description.tableofcontents | Ⅰ. Introduction 1 Ⅱ. Material and Methods 4 2.1 Cell culture 4 2.2 Triton X-100 fraction assay 4 2.3 Immunoprecipitation assay 5 2.4 Western blot 6 2.5 Thioflavin S staining 7 2.6 Immunocytochemical staining 8 2.7 RNA isolation and Quantitative Real Time Polymerase Chain Reaction (qRT PCR) 8 2.8 Mutagenesis of Cysteine 9 2.9 ROS assay 10 2.10 Glutaredoxin fluorometric activity assay 10 2.11 In vitro recombinant tau glutathionylation assay 11 2.12 TEM imaging 11 2.13 Statistical analysis 12 Ⅲ. Results 13 3.1 Development of a cellular model of templated aggregation of tau 13 3.2 Overexpression of GRX1 reduces the levels of soluble and insoluble tau in a cellular model of templated aggregation of tau 18 3.3 Downregulation of GRX1 increases the levels of soluble and insoluble tau in a cellular model of templated aggregation of tau 23 3.4 GRX1 regulates the ROS levels in a cellular model of templated aggregation of tau 26 3.5 Overexpression of GRX1 inhibits apoptosis in a cellular model of templated aggregation of tau 29 3.6 Tau is not occurred glutathionylation in cells 32 3.7 GRX1 may accelerate the degration pathway of P301S tau by autophagic lysosomal pathway 38 Ⅳ. Discussion 42 Ⅴ. Reference 45 Ⅵ. Abstract in Korean 50 |
- |
| dc.format.extent | 51 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Investigation of the protective role of Glutaredoxin 1 in the tauopathy through inhibiting aggregation of tau | - |
| dc.title.alternative | 타우 응집 억제를 통한 Glutaredoxin 1의 타우병증에서 보호적 역할 규명 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000658141 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | Department of Brain Sciences | - |
| dc.identifier.bibliographicCitation | Eunmi Lee. (2023). Investigation of the protective role of Glutaredoxin 1 in the tauopathy through inhibiting aggregation of tau. doi: 10.22677/THESIS.200000658141 | - |
| dc.contributor.coadvisor | Jaekwang Kim | - |
| dc.date.awarded | 2023-02-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.BM 이67 202302 | - |
| dc.date.accepted | 2023-03-21 | - |
| dc.contributor.alternativeDepartment | 뇌과학과 | - |
| dc.subject.keyword | Alzheimer's disease | - |
| dc.subject.keyword | Tauopathy | - |
| dc.subject.keyword | Glutaredoxin 1 | - |
| dc.subject.keyword | Templated aggregation of tau | - |
| dc.subject.keyword | Reactive oxygen species | - |
| dc.subject.keyword | Apoptosis | - |
| dc.subject.keyword | Autophagy | - |
| dc.contributor.affiliatedAuthor | Eunmi Lee | - |
| dc.contributor.affiliatedAuthor | Sung Bae Lee | - |
| dc.contributor.affiliatedAuthor | Jaekwang Kim | - |
| dc.contributor.alternativeName | 이은미 | - |
| dc.contributor.alternativeName | Sung Bae Lee | - |
| dc.contributor.alternativeName | 김재광 | - |
| dc.rights.embargoReleaseDate | 2026-02-28 | - |
