Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jaferzadeh, Keyvan | - |
dc.contributor.author | Rappaz, Benjamin | - |
dc.contributor.author | Kim, Youhyun | - |
dc.contributor.author | Kim, Bo-Kyung | - |
dc.contributor.author | Moon, Inkyu | - |
dc.contributor.author | Marquet, Pierre | - |
dc.contributor.author | Turcatti, Gerardo | - |
dc.date.accessioned | 2023-08-30T12:10:18Z | - |
dc.date.available | 2023-08-30T12:10:18Z | - |
dc.date.created | 2023-07-20 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/46350 | - |
dc.description.abstract | This manuscript proposes a new dual-mode cell imaging system for studying the relationships between calcium dynamics and the contractility process of cardiomyocytes derived from human-induced pluripotent stem cells. Practically, this dual-mode cell imaging system provides simultaneously both live cell calcium imaging and quantitative phase imaging based on digital holographic microscopy. Specifically, thanks to the development of a robust automated image analysis, simultaneous measurements of both intracellular calcium, a key player of excitation-contraction coupling, and the quantitative phase image-derived dry mass redistribution, reflecting the effective contractility, namely, the contraction and relaxation processes, were achieved. Practically, the relationships between calcium dynamics and the contraction-relaxation kinetics were investigated in particular through the application of two drugs─namely, isoprenaline and E-4031─known to act precisely on calcium dynamics. Specifically, this new dual-mode cell imaging system enabled us to establish that calcium regulation can be divided into two phases, an early phase influencing the occurrence of the relaxation process followed by a late phase, which although not having a significant influence on the relaxation process affects significantly the beat frequency. In combination with cutting-edge technologies allowing the generation of human stem cell-derived cardiomyocytes, this dual-mode cell monitoring approach therefore represents a very promising technique, particularly in the fields of drug discovery and personalized medicine, to identify compounds likely to act more selectively on specific steps that compose the cardiomyocyte contractility. © 2023 American Chemical Society. | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.title | Automated Dual-Mode Cell Monitoring To Simultaneously Explore Calcium Dynamics and Contraction-Relaxation Kinetics within Drug-Treated Stem Cell-Derived Cardiomyocytes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acssensors.3c00073 | - |
dc.identifier.wosid | 001011507800001 | - |
dc.identifier.scopusid | 2-s2.0-85164466981 | - |
dc.identifier.bibliographicCitation | ACS Sensors, v.8, no.7, pp.2533 - 2542 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | dual-modeimagingsystem | - |
dc.subject.keywordAuthor | multimodalbiosensors | - |
dc.subject.keywordAuthor | cardiomyocytesanalysis | - |
dc.subject.keywordAuthor | drug profiling | - |
dc.subject.keywordAuthor | cardiotoxicity | - |
dc.subject.keywordPlus | DIGITAL HOLOGRAPHIC MICROSCOPY | - |
dc.subject.keywordPlus | NUMERICAL RECONSTRUCTION | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ISOPRENALINE | - |
dc.subject.keywordPlus | FORCE | - |
dc.subject.keywordPlus | CONTRAST | - |
dc.subject.keywordPlus | TOOL | - |
dc.citation.endPage | 2542 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 2533 | - |
dc.citation.title | ACS Sensors | - |
dc.citation.volume | 8 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry; Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology | - |
dc.type.docType | Article | - |
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