Title

Acoustic Radiation Force Impulse System based on Ultra-fast Acoustic Tweezers for Measurement of Cell Mechanics

DGIST Authors

HeeYeon Park ; Jae Youn Hwang ; Jin Ho Chang

Advisor

황재윤

Co-Advisor(s)

Jin Ho Chang

Issued Date

2023

Awarded Date

2023-08-01

Citation

HeeYeon Park. (2023). Acoustic Radiation Force Impulse System based on Ultra-fast Acoustic Tweezers for Measurement of Cell Mechanics. doi: 10.22677/THESIS.200000688298

Type

Thesis

Description

Acoustic radiation force impulse system; Acoustic tweezer; Quantitative phase imaging; Cell mechanics; Poisson’s ratio; 음향 방사력 임펄스 시스템; 음향 핀셋; 정량적 위상 이미징; 세포 역학; 포아송 비

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Methods 7
2.1 High-frequency Ultrasound Transducer 7
2.2 Suspended Cell Preparation 9
2.3 Acoustic Radiation Force Impulse System based on Acoustic Tweezers 10
2.4 Acoustic Tweezers Actuation and Cell Trapping Mechanisms 13
2.5 Image Processing using MATLAB 15
2.6 Calculation of The Strain and Poisson’s Ratio of a Trapped Cell 17
Ⅲ. Results 18
3.1 Cancer Cell Trapping by Acoustic Radiation, and Measurement of Cell Deformation Over Time Under Parameter Conditions 18
3.2 Comparison of Cell Length and Thickness Variations of MCF-7 and MDA-MB-231 21
3.3 Calculation and Analysis of Poisson's Ratio of MCF-7 and MDA-MB-231 23
3.4 Comparison of Thickness and Length Measurements of MCF-7 and MDA-MB-231 at Each Acoustic Pressure and Their Corresponding Deformation 25
3.5 Measurement of Poisson's Ratio of MCF-7 and MDA-MB-231 at Each Acoustic Pressure 28
Ⅳ. Conclusion 31
Ⅴ. Discussion 37
References 40
국문요약문 42

URI

http://hdl.handle.net/20.500.11750/46442
http://dgist.dcollection.net/common/orgView/200000688298

DOI

10.22677/THESIS.200000688298

Degree

Master

Department

Department of Electrical Engineering and Computer Science

Publisher

DGIST

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