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Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy

Title
Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy
Author(s)
Lee, Bo RamJoo, Kyung-IlChoi, Eun SookJahng, JunghoonKim, Hyun MinKim, Eun Joo
Issued Date
2017-10
Citation
PLoS ONE, v.12, no.10
Type
Article
Keywords
CARS-MICROSCOPYLIPID DROPLETSIN-VIVOBARRIERSPECTROSCOPYPERMEABILITYRESOLUTIONCELLSLASER
ISSN
1932-6203
Abstract
We performed dye-enhanced imaging of mouse brain microvessels using spectral focusing coherent anti-Stokes Raman scattering (SF-CARS) microscopy. The resonant signals from C-H stretching in forward CARS usually show high background intensity in tissues, which makes CARS imaging of microvessels difficult. In this study, epi-detection of back-scattered SF-CARS signals showed a negligible background, but the overall intensity of resonant CARS signals was too low to observe the network of brain microvessels. Therefore, Evans blue (EB) dye was used as contrasting agent to enhance the back-scattered SF-CARS signals. Breakdown of brain microvessels by inducing hemorrhage in a mouse was clearly visualized using backward SF-CARS signals, following intravenous injection of EB. The improved visualization of brain microvessels with EB enhanced the sensitivity of SF-CARS, detecting not only the blood vessels themselves but their integrity as well in the brain vasculature. © 2017 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI
http://hdl.handle.net/20.500.11750/4752
DOI
10.1371/journal.pone.0185519
Publisher
Public Library of Science
Related Researcher
  • 김현민 Kim, Hyunmin
  • Research Interests Nonlinear optics; Femtosecond ultrafast carrier dynamics; Super resolution microscopy; Optical soliton transport; Biological imaging
Files in This Item:
10.1371_journal.pone.0185519.pdf

10.1371_journal.pone.0185519.pdf

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Appears in Collections:
Division of Biomedical Technology 1. Journal Articles
Companion Diagnostics and Medical Technology Research Group 1. Journal Articles
Division of AI, Big data and Block chain 1. Journal Articles

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