Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lee, Yoonhee | - |
dc.contributor.author | Buchheim, Jakob | - |
dc.contributor.author | Hellenkamp, Bjorn | - |
dc.contributor.author | Lynall, David | - |
dc.contributor.author | Yang, Kyungae | - |
dc.contributor.author | Young, Erik F. | - |
dc.contributor.author | Penkov, Boyan | - |
dc.contributor.author | Sia, Samuel | - |
dc.contributor.author | Stojanovic, Milan N. | - |
dc.contributor.author | Shepard, Kenneth L. | - |
dc.date.accessioned | 2024-02-07T22:40:10Z | - |
dc.date.available | 2024-02-07T22:40:10Z | - |
dc.date.created | 2024-02-01 | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 1748-3387 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/47862 | - |
dc.description.abstract | Small molecules such as neurotransmitters are critical for biochemical functions in living systems. While conventional ultraviolet–visible spectroscopy and mass spectrometry lack portability and are unsuitable for time-resolved measurements in situ, techniques such as amperometry and traditional field-effect detection require a large ensemble of molecules to reach detectable signal levels. Here we demonstrate the potential of carbon-nanotube-based single-molecule field-effect transistors (smFETs), which can detect the charge on a single molecule, as a new platform for recognizing and assaying small molecules. smFETs are formed by the covalent attachment of a probe molecule, in our case a DNA aptamer, to a carbon nanotube. Conformation changes on binding are manifest as discrete changes in the nanotube electrical conductance. By monitoring the kinetics of conformational changes in a binding aptamer, we show that smFETs can detect and quantify serotonin at the single-molecule level, providing unique insights into the dynamics of the aptamer–ligand system. In particular, we show the involvement of G-quadruplex formation and the disruption of the native hairpin structure in the conformational changes of the serotonin–aptamer complex. The smFET is a label-free approach to analysing molecular interactions at the single-molecule level with high temporal resolution, providing additional insights into complex biological processes. © 2024, The Author(s), under exclusive licence to Springer Nature Limited. | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Carbon-nanotube field-effect transistors for resolving single-molecule aptamer-ligand binding kinetics | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41565-023-01591-0 | - |
dc.identifier.scopusid | 2-s2.0-85182429717 | - |
dc.identifier.bibliographicCitation | Nature Nanotechnology | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | NEUROTRANSMITTER | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | DOPAMINE | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | BRAIN | - |
dc.citation.title | Nature Nanotechnology | - |
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