Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lim, Heejin | - |
dc.contributor.author | Lee, Siheun | - |
dc.contributor.author | Jin, Jong Sung | - |
dc.contributor.author | Kim, Min-Sik | - |
dc.date.accessioned | 2024-02-26T18:10:16Z | - |
dc.date.available | 2024-02-26T18:10:16Z | - |
dc.date.created | 2024-02-22 | - |
dc.date.issued | 2024-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/47984 | - |
dc.description.abstract | Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has become a promising analytical tool for molecular profiling in biological applications. However, its ultrahigh vacuum environment and matrix effects hamper the absolute quantitation of solution samples. Herein, we present a rapid high-throughput platform for quantitative ToF-SIMS analysis of amino acids in matrix deposits formed from freeze-dried solution drops through ice sublimation on a parylene film microarray substrate. Droplets of the amino acid solutions, which were mixed with stable isotope-labeled phenylalanine (F*) of high concentration (10 mM), were loaded on wells of the microarray, then frozen and evaporated slowly below the freezing point, forming continuous solid-phase F* matrix deposits. The amino acids (≤500 μM), adequately well dispersed throughout the F* matrix deposits on each well, were quantitatively analyzed by ToF-SIMS in a rapid and high-throughput fashion. The lower limit of quantitation reached below 10 μM. © 2024 American Chemical Society. | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.title | High-Throughput Quantitative Analysis of Amino Acids in Freeze-Dried Drops Using Time-of-Flight Secondary Ion Mass Spectrometry | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.3c04855 | - |
dc.identifier.wosid | 001162204200001 | - |
dc.identifier.scopusid | 2-s2.0-85184863909 | - |
dc.identifier.bibliographicCitation | Analytical Chemistry, v.96, no.9, pp.3717 - 3721 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordPlus | TOF-SIMS DATA | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | BEAMS | - |
dc.citation.endPage | 3721 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 3717 | - |
dc.citation.title | Analytical Chemistry | - |
dc.citation.volume | 96 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.type.docType | Article | - |
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