Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 서진수 | - |
| dc.contributor.author | Na Yeon Kim | - |
| dc.date.accessioned | 2024-02-29T21:01:23Z | - |
| dc.date.available | 2024-02-29T21:01:23Z | - |
| dc.date.issued | 2024 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/48051 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000724600 | - |
| dc.description | Alzheimer's disease;Priming;Astrocytes;Microglia;Apolipoprotein E;Aβ | - |
| dc.description.tableofcontents | Ⅰ. Introduction 1 1.1 Neuroinflammatory environment in AD 1 1.2 Innate immune memory : priming effect 2 1.3 Apolipoprotein E4 (ApoE4) and AD 3 1.4 Human-induced pluripotent stem cell model (hiPSC) 3 Ⅱ. Materials and Methods 5 2.1 Cell culture 5 2.1.1 Human-induced pluripotent stem cell (hiPSC) 5 2.1.2 Neural progenitor cells (NPCs) differentiation 5 2.1.3 Astrocyte differentiation 6 2.1.4 Immortalized human microglia 6 2.2 Immune challenge and induction of priming 6 2.3 RNA extraction and cDNA synthesis 6 2.4 quantitative Real-time PCR (qRT-PCR) 7 2.5 Immunocytochemistry 7 2.6 Immunohistochemistry 8 2.7 Cytokine array analysis 8 2.8 Aβ42 uptake assay 9 2.9 Microglial Aβ42 clearance assay 9 2.10 Statistical analysis 10 Ⅲ. Results 11 3.1 Generation of isogenic hiPSC-derived astrocytes and characterization of their identify 11 3.2 Probing priming effect of hiPSC-derived astrocytes by the immune challenge 14 3.3 Aβ42-treated primed astrocytes upregulate the immune response 17 3.4 Aβ42-treated primed astrocytes induce enhancement of Aβ42 clearance of human microglia 20 3.5 APOE4 astrocytes have a priming effect 22 3.6 Primed APOE4 astrocytes enhance Aβ42 uptake but reduce immune response compared with primed APOE3 astrocytes 23 3.7 Aβ42-treated primed APOE4 astrocytes reduce Aβ42 clearance of human microglia 26 3.8 A 9-week-old wild-type (C57BL/6J) male mouse has a priming effect, as in vitro 29 3.9 Confirming the priming effect and Aβ42 clearance of the 7-month-old humanized APOEKI 5XFAD male mouse 33 3.10 Confirming the priming effect and Aβ42 clearance of the 6-month-old humanized APOE KI male mouse 41 Ⅵ. Discussion 44 Ⅴ. References 47 Abstract in Korean 50 List of tables and figures Table 1. Information of cell lines used in the experiment Figure 1. Generation of the isogenic hiPSC-derived astrocytes and confirmation of their identify Figure 2. hiPSC-derived astrocytes have a priming effect Figure 3. Aβ42-treated primed astrocytes upregulate the immune response Figure 4. The effect of primed astrocytes on microglia in AD context Figure 5. APOE4 astrocytes have a priming effect and Aβ42-treated primed APOE4 astrocytes reduce immune response compared with Aβ42-treated primed APOE3 astrocytes Figure 6. The effect of primed APOE4 astrocytes on microglia in AD context Figure 7. A wild-type male mouse astrocyte has a priming effect Figure 8. Confirming the priming effect and Aβ42 clearance of the humanized APOE KI 5XFAD male mouse Figure 9. Confirming the priming effect and Aβ42 clearance of the humanized APOE KI male mouse Figure 10. Graphic Summary |
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| dc.format.extent | 50 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Investigating effects of primed astrocytes on microglia in Alzheimer’s disease pathology | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000724600 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | Department of Brain Sciences | - |
| dc.contributor.coadvisor | Seong-Woon Yu | - |
| dc.date.awarded | 2024-02-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.BM김19 202402 | - |
| dc.date.accepted | 2024-01-30 | - |
| dc.contributor.alternativeDepartment | 뇌과학과 | - |
| dc.subject.keyword | Alzheimer's disease | - |
| dc.subject.keyword | Priming | - |
| dc.subject.keyword | Astrocytes | - |
| dc.subject.keyword | Microglia | - |
| dc.subject.keyword | Apolipoprotein E | - |
| dc.subject.keyword | Aβ | - |
| dc.contributor.affiliatedAuthor | Na Yeon Kim | - |
| dc.contributor.affiliatedAuthor | Jinsoo Seo | - |
| dc.contributor.affiliatedAuthor | Seong-Woon Yu | - |
| dc.contributor.alternativeName | 김나연 | - |
| dc.contributor.alternativeName | Jinsoo Seo | - |
| dc.contributor.alternativeName | 유성운 | - |
| dc.rights.embargoReleaseDate | 2026-02-28 | - |
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