Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Dong Jae | ko |
dc.contributor.author | Kang, Min-Jung | ko |
dc.contributor.author | Choi, Jin-A | ko |
dc.contributor.author | Na, Dae-Seung | ko |
dc.contributor.author | Kim, Jin-Beom | ko |
dc.contributor.author | Na, Chun-Soo | ko |
dc.contributor.author | Park, Jong-Hwan | ko |
dc.date.accessioned | 2018-01-24T02:01:07Z | - |
dc.date.available | 2018-01-24T02:01:07Z | - |
dc.date.created | 2018-01-17 | - |
dc.date.issued | 2016-06 | - |
dc.identifier.citation | Laboratory Animal Research, v.32, no.2, pp.99 - 104 | - |
dc.identifier.issn | 1738-6055 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/4887 | - |
dc.description.abstract | Helicobacter pylori colonizes the gastric mucosa of about half of the world’s population, causing chronic gastritis and gastric cancer. An increasing emergence of antibiotic-resistant H. pylori arouses demand on alternative non-antibiotic-based therapies. In this study, we freshly prepared crude N-acetylneuraminic acid obtained from glycomacropeptide (G-NANA) of whey through a neuraminidase-mediated reaction and evaluated its antibacterial ability against H. pylori and H. felis. Overnight cultures of the H. pylori were diluted with fresh media and different concentrations (1-150 mg/mL) of crude G-NANA were added directly to the culture tube. Bacterial growth was evaluated by measuring the optical density of the culture medium and the number of viable bacteria was determined by a direct count of the colony forming units (CFU) on agar plates. For the in vivo study, mice were orally infected with 100 µL (5×108 cfu/mL) of H. felis four times at a day’s interval, accompanied by a daily administration of crude G-NANA or vehicle. A day after the last infection, the mice were daily administered the crude G-NANA (0, 75, and 300 mg/mL) for 10 days and euthanized. Their stomachs were collected and bacterial colonization was determined by quantitative real-time PCR. Crude G-NANA inhibited H. pylori’s growth and reduced the number of viable bacteria in a dose-dependent manner. Furthermore, crude G-NANA inhibited bacterial colonization in the mice. These results showed that crude G-NANA has antibacterial activity against Helicobacter and demonstrated its therapeutic potential for the prevention of chronic gastritis and gastric carcinogenesis induced by Helicobacter infection in humans. | - |
dc.language | English | - |
dc.publisher | 한국실험동물학회 | - |
dc.subject | N-neuraminic acid | - |
dc.subject | glycomacropeptide | - |
dc.subject | Helicobacter | - |
dc.subject | antibacterial activity | - |
dc.title | Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey | - |
dc.type | Article | - |
dc.identifier.doi | 10.5625/lar.2016.32.2.99 | - |
dc.type.local | Article(Domestic) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.kciid | ART002122259 | - |
dc.contributor.nonIdAuthor | Kang, Min-Jung | - |
dc.contributor.nonIdAuthor | Choi, Jin-A | - |
dc.contributor.nonIdAuthor | Na, Dae-Seung | - |
dc.contributor.nonIdAuthor | Kim, Jin-Beom | - |
dc.contributor.nonIdAuthor | Na, Chun-Soo | - |
dc.contributor.nonIdAuthor | Park, Jong-Hwan | - |
dc.identifier.citationVolume | 32 | - |
dc.identifier.citationNumber | 2 | - |
dc.identifier.citationStartPage | 99 | - |
dc.identifier.citationEndPage | 104 | - |
dc.identifier.citationTitle | Laboratory Animal Research | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Dong Jae | - |
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