Cited 4 time in webofscience Cited 3 time in scopus

Reverse Expression of Aging-Associated Molecules through Transfection of miRNAs to Aged Mice

Title
Reverse Expression of Aging-Associated Molecules through Transfection of miRNAs to Aged Mice
Authors
Kim, Jung-HeeLee, Bo-RamChoi, Eun-SookLee, Kyeong-MinChoi, Seong-KyoonCho, Jung HoonJeon, Won BaeKim, Eunjoo
DGIST Authors
Lee, Kyeong-MinJeon, Won BaeKim, Eunjoo
Issue Date
2017-03-17
Citation
Molecular Therapy - Nucleic Acids, 6, 106-115
Type
Article
Article Type
Article
Keywords
Aging BiomarkerBiomarkerCancerCellular SenescenceCirculating MirnaDiseaseMacrophagesMicroRNAsMouseNon Alcoholic Fatty Liver DiseasePathwayProliferationReverse AgingShort TelomeresTelomerase
ISSN
2162-2531
Abstract
Molecular changes during aging have been studied to understand the mechanism of aging progress. Herein, changes in microRNA (miRNA) expression in the whole blood of mice were studied to systemically reverse aging and propose them as non-invasive biomarkers. Through next-generation sequencing analysis, we selected 27 differentially expressed miRNAs during aging. The most recognized function involved was liver steatosis, a type of non-alcoholic fatty liver disease (NAFLD). Among 27 miRNAs, six were predicted to be involved in NAFLD, miR-16-5p, miR-17-5p, miR-21a-5p, miR-30c-5p, miR-103-3p, and miR-130a-3p; alterations in their blood and liver levels were confirmed by real-time qPCR. The expression of the genes associated in the network of these miRNAs, Bcl2, Ppara, E2f1, E2f2, Akt, Ccnd1, and Smad2/3, also was altered in the liver of aged mice. Following transfection of these miRNAs into 18-month-old mice, levels of miR-21a-5p, miR-103-3p, and miR-30c-5p increased, and their related genes exhibited a reversed expression in the liver. Expression of Mre11a, p16INK4a, and Mtor, reported to be aging-associated molecules, also was reversed in the livers of miRNA-transfected mice. These miRNAs could be non-invasive biomarkers for aging, and they might induce a reverse regulation of aging-associated pathways. This study provides preliminary data on reverse aging, which could be applied further for treatments of adult diseases. © 2016 The Author(s)
URI
http://hdl.handle.net/20.500.11750/5013
DOI
10.1016/j.omtn.2016.11.005
Publisher
Elsevier Inc
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, nanomedicine, molecular diagnosis
Files:
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Collection:
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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