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A systematic study of nuclear interactome of C-terminal domain small phosphatase-like 2 using inducible expression system and shotgun proteomics

Title
A systematic study of nuclear interactome of C-terminal domain small phosphatase-like 2 using inducible expression system and shotgun proteomics
Authors
Kang, NanaKoo, JaeHyungWang, SenHur, Sun JinBahk, Young Yil
DGIST Authors
Koo, JaeHyung
Issue Date
2016-06-30
Citation
BMB Reports, 49(6), 319-324
Type
Article
Article Type
Article
Keywords
CTD PhosphataseCTDSPL2CTDSPL2 Inducible HEK293T Cell LineImmunoprecipitationNuclear Interactome
ISSN
1976-6696
Abstract
RNA polymerase II C-terminal domain phosphatases are newly emerging family of phosphatases that contain FCPH domain with Mg+2-binding DXDX(T/V) signature motif. Its subfamily includes small CTD phosphatases (SCPs). Recently, we identified several interacting partners of human SCP1 with appearance of dephosphorylation and O-GlcNAcylation. In this study, using an established cell line with inducible CTDSPL2 protein (a member of the new phosphatase family), proteomic screening was conducted to identify binding partners of CTDSPL2 in nuclear extract through immunoprecipitation of CTDSPL2 with its associated. This approach led to the identification of several interacting partners of CTDSPL2. This will provide a better understanding on CTDSPL2. © 2016 by the The Korean Society for Biochemistry and Molecular Biology.
URI
http://hdl.handle.net/20.500.11750/5100
DOI
10.5483/BMBRep.2016.49.6.240
Publisher
Korean Society for Molecular and Cellular Biology
Related Researcher
  • Author Koo, JaeHyung Molecular & Cellular Smell Biology Lab
  • Research Interests Neurotranslation and chemosensation; 화학감각신경(후각, 미각) 및 이소성후각수용체 연구와 응용
Files:
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Collection:
Department of Brain and Cognitive SciencesMolecular & Cellular Smell Biology Lab1. Journal Articles


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