Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis
- Title
- Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis
- Author(s)
- Kim, Sung-Hee ; Yoon, Yeo Cho ; Lee, Ae Sin ; Kang, NaNa ; Koo, JaeHyung ; Rhyu, Mee-Ra ; Park, Jae-Ho
- DGIST Authors
- Koo, JaeHyung
- Issued Date
- 2015-05-01
- Type
- Article
- Article Type
- Article
- Subject
- AKT ; Angiogenesis ; Animal ; Animals ; Aorta ; C57Bl Mouse ; Calcium ; Coronary Blood Vessel ; Coronary Vessels ; Endothelium, Vascular ; Enzymology ; Extracellular Signal-Regulated Map Kinases ; Gene Knockdown Techniques ; Gene Silencing ; Human ; Human Umbilical Vein Endothelial Cells ; Humans ; HUVEC ; Male ; Metabolism ; Mice ; Mice, Inbred C57BL ; Mitogen-Activated Protein Kinase ; Mouse ; Neo-Vascularization, Physiologic ; Olfactory Receptor ; OR10J5 ; OR10J5 Protein, Human ; Phosphorylation ; Physiology ; Protein Kinase B ; Proto-Oncogene Proteins C-AKT ; Receptors, Odorant ; Umbilical Vein Endothelial Cell ; Vascular Endothelium
- ISSN
- 0006-291X
- Abstract
- ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca2+ and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca2+ and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells. © 2015 Elsevier Inc. All rights reserved.
- Publisher
- Academic Press Inc.
- Related Researcher
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Koo, JaeHyung 뉴바이올로지학과
- Research Interests 장내미생물/감염균 유래 대사체를 통한 신경염증; 알츠하이머병; 우울증; 당뇨/비만; 대사체/수용체 상호작용에 의한 대사연구
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- Appears in Collections:
- Department of New Biology Brain-Immune Axis Laboratory 1. Journal Articles
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