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Mouse neutrophils express functional umami taste receptor T1R1/T1R3
- Mouse neutrophils express functional umami taste receptor T1R1/T1R3
- Lee, NaHye; Jung, Young Su; Lee, Ha Young; Kang, NaNa; Park, Yoo Jung; Hwang, Jae Sam; Bahk, Young Yil; Koo, JaeHyung; Bae, Yoe-Sik
- DGIST Authors
- Koo, JaeHyung
- Issue Date
- BMB Reports, 47(11), 649-654
- Article Type
- Alanine; Animal; Animals; Antagonists and Inhibitors; Calcium; Cell Motion; Cell Movement; Chemistry; Chemotaxis; Cytokine; Cytokines; Cytology; Drug Effects; G Protein-Coupled Receptor; Gene Expression Regulation; Genetics; Immunoglobulin Enhancer Binding Protein; Inflammation; Isolation and Purification; Lipopolysaccharide; Lipopolysaccharides; Metabolism; Mice; Mouse; Neutrophil; Neutrophils; NF-Kappa B; Phosphorylation; Receptors, G-Protein-Coupled; RNA; Sequence Analysis; Sequence Analysis, RNA; Serine; Signal Transduction; STAT3 Protein; STAT3 Transcription Factor; Taste Receptors, Type1; Tumor Necrosis Factor-Alpha; Umami Taste Receptor T1R1/T1R3
- Neutrophils play an important role in the initiation of innate immunity against infection and injury. Although many different types of G-protein coupled receptors are functionally expressed in neutrophils, no reports have demonstrated functional expression of umami taste receptor in these cells. We observed that mouse neutrophils express the umami taste receptor T1R1/T1R3 through RNA sequencing and quantitative RT-PCR analysis. Stimulation of mouse neutrophils with L-alanine or L-serine, which are ligands for the umami taste receptor, elicited not only ERK or p38 MAPK phosphorylation but also chemotactic migration. Moreover, addition of L-alanine or L-serine markedly reduced the production of several cytokines including TNF-α induced by lipopolysaccharide (LPS) through inhibition of NF-κB activity or STAT3 phosphorylation in neutrophils. Our findings demonstrate that neutrophils express the umami taste receptor, through which tastants stimulate neutrophils, resulting in chemotactic migration, and attenuation of LPS-induced inflammatory response. © 2014 by the The Korean Society for Biochemistry and Molecular Biology.
- Korean Society for Biochemistry and Molecular Biology
- Related Researcher
Molecular & Cellular Smell Biology Lab
Neurotranslation and chemosensation; 화학감각신경(후각, 미각) 및 이소성후각수용체 연구와 응용
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- Department of New BiologyMolecular & Cellular Smell Biology Lab1. Journal Articles
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