Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Jae Young | - |
dc.contributor.author | Seo, Eun Seok | - |
dc.contributor.author | Kim, Hyun Min | - |
dc.contributor.author | Park, Ji Won | - |
dc.contributor.author | Lim, Dong Kwon | - |
dc.contributor.author | Moon, Dae Won | - |
dc.date.available | 2018-02-05T04:11:56Z | - |
dc.date.created | 2018-01-18 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5619 | - |
dc.description.abstract | We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 μm was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl β-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41467-017-02216-6 | - |
dc.identifier.scopusid | 2-s2.0-85038433296 | - |
dc.identifier.bibliographicCitation | Nature Communications, v.8, no.0, pp.2113 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | FLOW-ASSISTED IONIZATION | - |
dc.subject.keywordPlus | ELECTROSPRAY-IONIZATION | - |
dc.subject.keywordPlus | AMBIENT CONDITIONS | - |
dc.subject.keywordPlus | CHEMICAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | QUANTITATIVE-ANALYSIS | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | DENTATE GYRUS | - |
dc.subject.keywordPlus | ION-SOURCE | - |
dc.subject.keywordPlus | REAL-TIME | - |
dc.subject.keywordPlus | TOF-SIMS | - |
dc.identifier.url | https://www.nature.com/articles/s41467-017-02216-6 | - |
dc.citation.number | 0 | - |
dc.citation.startPage | 2113 | - |
dc.citation.title | Nature Communications | - |
dc.citation.volume | 8 | - |