Title

Development of micro and nanorobots using magnetic nanoparticles for targeted cell and drug delivery to treat eye disease and brain tumors

Alternative Title

자성나노입자를 이용한 안구 질환 및 뇌 종양의 세포 및 약물 치료를 위한 마이크로 나노로봇의 개발

DGIST Authors

Seungmin Noh ; Hongsoo Choi ; Yong-Seok Oh

Advisor

최홍수

Co-Advisor(s)

Yong-Seok Oh

Issued Date

2024

Awarded Date

2024-08-01

Citation

Seungmin Noh. (2024). Development of micro and nanorobots using magnetic nanoparticles for targeted cell and drug delivery to treat eye disease and brain tumors. doi: 10.22677/THESIS.200000798105

Type

Thesis

Description

Micro/nanorobots, magnetic nanoparticles, Chimeric antigen receptor (CAR) T cells, biodegradable microrobot.

Table Of Contents

Ⅰ. Introduction 1
1.1 Background 1
1.2 Research trends 2
1.2.1 Microrobots with various energy source 2
1.2.2 Magnetic microrobots for targeted therapy 5
1.3 Objective in this research 10
ⅠI. A biodegradable magnetic microrobot based on gelatin methacrylate for precise delivery
of stem cells with mass production capability. 13
2.1 Introduction 13
2.2 Experimental result 17
2.2.1 Fabrication of GelMA-based droplet microrobots 17
2.2.2 Stem cell loaded microrobots 20
2.2.3 Magnetic manipulation of microrobots 23
2.2.4 Enzymatic degradation of the microrobots 27
2.2.5 Electrophysiological activity of delivered neurons 30
2.3 Discussion & Conclusion 31
2.4 Materials & Methods 32
2.4.1 Fabrication of the flow-focusing droplet microfluidic device 32
2.4.2 Fabrication of the GelMA-based biodegradable microrobot 33
IⅠI. Magnetically controlled intraocular delivery of dexamethasone using silica-coated
magnetic nanoparticles. 35
3.1 Introduction 35
3.2 Experimental result 39
3.2.1 Fabrication of the magnetic nanoparticles 39
3.2.2 Biocompatibility of SMNPs 41
3.2.3 Dexamethasone loading on SMNPs 43
3.2.4 Dexamethasone release from SMNPs 45
3.2.5 Magnetic manipulation of Dex@MNPs inside the vitreous humor 48
3.2.6 Animal experiment 49
3.3 Conclusion 51
3.4 Materials & Methods 52
3.4.1 Dexamethasone loading on SMNPs 52
3.4.2 Animal experiment 53
IV. Epithermal growth factor targeted chimeric antigen receptor (CAR) T cell bots for
improved tissue infiltration and precise magnetic manipulation. 54
4.1 Introduction 54
4.2 Experimental result 57
4.2.1 Fabrication of CAR T cellbots 58
4.2.2 Characteristics of CAR T cellbots 61
4.2.3 Cytotoxicity of CAR T cellbots 64
4.2.4 Magnetic manipulation of CAR T cellbots 67
4.2.5 Magnetic manipulation of CAR T cellbots in in vivo 69
4.3 Discussion & Conclusion 71
V. Conclusion 73
5.1 Overall conclusion 73
5.2 Future work 75
5.2.1 A biodegradable magnetic microrobot based on gelatin methacrylate for precise
delivery of stem cells with mass production capability 75
5.2.2 Magnetically controlled intraocular delivery of dexamethasone using silica-coated
magnetic nanoparticles 76
5.2.3 Epithermal growth factor targeted chimeric antigen receptor (CAR) T cell bots for
improved tissue infiltration and precise magnetic manipulation 77
Acknowledgement 78
Reference 80
요약문 98
Appendix 101

URI

http://hdl.handle.net/20.500.11750/57576
http://dgist.dcollection.net/common/orgView/200000798105

DOI

10.22677/THESIS.200000798105

Degree

Doctor

Department

Department of Robotics and Mechatronics Engineering

Publisher

DGIST

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