Seungmin Noh. (2024). Development of micro and nanorobots using magnetic nanoparticles for targeted cell and drug delivery to treat eye disease and brain tumors. doi: 10.22677/THESIS.200000798105
Type
Thesis
Description
Micro/nanorobots, magnetic nanoparticles, Chimeric antigen receptor (CAR) T cells, biodegradable microrobot.
Table Of Contents
Ⅰ. Introduction 1 1.1 Background 1 1.2 Research trends 2 1.2.1 Microrobots with various energy source 2 1.2.2 Magnetic microrobots for targeted therapy 5 1.3 Objective in this research 10 ⅠI. A biodegradable magnetic microrobot based on gelatin methacrylate for precise delivery of stem cells with mass production capability. 13 2.1 Introduction 13 2.2 Experimental result 17 2.2.1 Fabrication of GelMA-based droplet microrobots 17 2.2.2 Stem cell loaded microrobots 20 2.2.3 Magnetic manipulation of microrobots 23 2.2.4 Enzymatic degradation of the microrobots 27 2.2.5 Electrophysiological activity of delivered neurons 30 2.3 Discussion & Conclusion 31 2.4 Materials & Methods 32 2.4.1 Fabrication of the flow-focusing droplet microfluidic device 32 2.4.2 Fabrication of the GelMA-based biodegradable microrobot 33 IⅠI. Magnetically controlled intraocular delivery of dexamethasone using silica-coated magnetic nanoparticles. 35 3.1 Introduction 35 3.2 Experimental result 39 3.2.1 Fabrication of the magnetic nanoparticles 39 3.2.2 Biocompatibility of SMNPs 41 3.2.3 Dexamethasone loading on SMNPs 43 3.2.4 Dexamethasone release from SMNPs 45 3.2.5 Magnetic manipulation of Dex@MNPs inside the vitreous humor 48 3.2.6 Animal experiment 49 3.3 Conclusion 51 3.4 Materials & Methods 52 3.4.1 Dexamethasone loading on SMNPs 52 3.4.2 Animal experiment 53 IV. Epithermal growth factor targeted chimeric antigen receptor (CAR) T cell bots for improved tissue infiltration and precise magnetic manipulation. 54 4.1 Introduction 54 4.2 Experimental result 57 4.2.1 Fabrication of CAR T cellbots 58 4.2.2 Characteristics of CAR T cellbots 61 4.2.3 Cytotoxicity of CAR T cellbots 64 4.2.4 Magnetic manipulation of CAR T cellbots 67 4.2.5 Magnetic manipulation of CAR T cellbots in in vivo 69 4.3 Discussion & Conclusion 71 V. Conclusion 73 5.1 Overall conclusion 73 5.2 Future work 75 5.2.1 A biodegradable magnetic microrobot based on gelatin methacrylate for precise delivery of stem cells with mass production capability 75 5.2.2 Magnetically controlled intraocular delivery of dexamethasone using silica-coated magnetic nanoparticles 76 5.2.3 Epithermal growth factor targeted chimeric antigen receptor (CAR) T cell bots for improved tissue infiltration and precise magnetic manipulation 77 Acknowledgement 78 Reference 80 요약문 98 Appendix 101
