Kim Seunghye. (2024). Investigation of MDGA2-mediated regulation of EphB2 synaptic function at excitatory synapses. doi: 10.22677/THESIS.200000798470
Type
Thesis
Description
MDGA2, EphB2, excitatory synapses, NMDAR, ephrin
Table Of Contents
I. Introduction 1.1 Synapses play a crucial role in the transfer of impulses between neurons 1 1.2 Synaptic adhesion molecules (SAMs) are important for maintaining synaptic function 2 1.3 Abnormalities of SAMs can lead to neuropsychiatric diseases 3 1.4 MDGA suppresses synaptic development through its interaction with Nlgn 4 1.5 MDGA regulates the function of excitatory postsynapses 5 1.6 EphB2 and ephrin proteins regulate the function of NMDAR 8
II. Materials and Methods 2.1 Animals 11 2.2 Expression Vectors 12 2.3 Cell culture, transfection, infection 13 2.4 Surface Binding Assay 14 2.5 Production of recombinant Ig-fusion proteins 15 2.6 Cell adhesion assay 16 2.7 Coimmunoprecipitation assay 16 2.8 Western Blot 17 2.9 Quantitative reverse transcription-polymerase chain reaction 19 2.10 AAV virus preparation 19 2.11 Stereotaxic injection 21 2.12 Mouse behavioral test 21 2.13 Data analysis 25
III. Results 3.1 Deletion of MDGA2 did not affect synaptic factors in cultured neurons 26 3.2 MDGA2 deletion alters mouse synaptic function in vivo 26 3.3 MDGA2 interacts with EphB2 28 3.4 MDGA2 exhibits calcium-independent binding to EphB2 and EphA7 within the Eph family while showing no binding to other RTKs 29 3.5 EphB2 forms cis-binding with MDGA2 and associates with MDGA2 In vivo 31 3.6 MDGA2 binds to the site where EphB2 interacts with ephrin proteins 32 3.7 Modulating EphB2 expression leads to the regulation of mouse behavior 33
IV. Discussion 37 V. Figure 41 VI. Reference 57 VII. 요약문 62
