Title

Keap1 regulates NK cells’ cytotoxicity in lung adenocarcinoma

Alternative Title

폐 선암에서 Keap1 조절을 통한 자연 살해 세포의 세포 독성 향상

DGIST Authors

Jeongmin Kim ; Youngtae Jeong ; Il-Kyu Choi

Advisor

정영태

Co-Advisor(s)

Il-Kyu Choi

Issued Date

2024

Awarded Date

2024-08-01

Citation

Jeongmin Kim. (2024). Keap1 regulates NK cells’ cytotoxicity in lung adenocarcinoma. doi: 10.22677/THESIS.200000798027

Type

Thesis

Description

Keap1, Natural Killer cells, Organoid, Co-culture, Cytotoxicity

Table Of Contents

Ⅰ. Introduction 1
1.1 Cancer and immunosurveillance 1
1.2 Cancer immunotherapy 2
1.3 Natural Killer cells 4
1.4 KEAP1 protein 5
Ⅱ. Materials and Methods 7
2.1 Mouse NK cells isolation and culture 7
2.2 Organoid and on-top seeded P/K-LUAD organoid culture 8
2.3 RT-qPCR of Keap1, Granzyme B and Perforin 1 9
2.4 IFN-γ production assay 10
2.5 Fluorescence In Vitro imaging 11
2.6 Cytotoxicity assay 11
2.7 statistical analysis 12
Ⅲ. Result 13
3.1 Isolation murine NK cells 13
3.2 Assessment of Granzyme B, Perforin1 and IFN-γ expression level 13
3.3 Establishment of on-top seeded P/K-LUAD organoids 14
3.4 Evaluation of NK cells’ cytotoxicity 15
3.5 Keap1 deletion enhances NK cell cytotoxicity with cytotoxic molecules 16
Ⅳ. Discussion 23
Reference 27
요약문 32

URI

http://hdl.handle.net/20.500.11750/57608
http://dgist.dcollection.net/common/orgView/200000798027

DOI

10.22677/THESIS.200000798027

Degree

Master

Department

Department of New Biology

Publisher

DGIST

Show Full Item Record