Title

FAT1 regulates head and neck cancer proliferation and migration via autophagy and mitochondrial dysfunction

DGIST Authors

Seongsoo Kim ; Youngtae Jeong ; Chang-Hun Lee

Advisor

정영태

Co-Advisor(s)

Chang-Hun Lee

Issued Date

2024

Awarded Date

2024-08-01

Citation

Seongsoo Kim. (2024). FAT1 regulates head and neck cancer proliferation and migration via autophagy and mitochondrial dysfunction. doi: 10.22677/THESIS.200000799197

Type

Thesis

Description

FAT1, HNSCC, Autophagy, Mitochondria, Stress, Apoptosis

Table Of Contents

1. Introduction 1
1.1. Development of HNSCC 1
1.2. FAT1 2
1.3. FAT1 in diverse cancer 4
1.3.1. Tumor suppressor 4
1.3.2. Tumor promoter 5
1.4. FAT1 in HNSCC 6
1.4.1. FAT1 as a biomarker of HNSCC 6
1.4.2. FAT1 as a potential tumor suppressor gene 6
1.4.3. FAT1 as a potential tumor promoter gene 7
1.5. Development of Autophagy 8
2. Materials and methods 11
2.1. Cell lines 11
2.2. Small interfering RNA 11
2.3. Cell proliferation assay 12
2.4. Colony formation assay 13
2.5. Wound scratch assay 13
2.6. Western blot assay 13
2.7. Antibodies 14
3. Results 16
3.1. FAT1 silencing inhibits cell proliferation and colony formation of HNSCC 16
3.2. FAT1 silencing inhibits cell migration of HNSCC 16
3.3. FAT1 silencing attenuates ULK1 dependent autophagy in HNSCC 17
3.4. FAT1 silencing induces mitochondria dysfunction and leads to HNSCC apoptosis 18
4. Discussion 29
References 33
요 약 문 36

URI

http://hdl.handle.net/20.500.11750/57646
http://dgist.dcollection.net/common/orgView/200000799197

DOI

10.22677/THESIS.200000799197

Degree

Master

Department

Department of New Biology

Publisher

DGIST

Show Full Item Record