Title

Deep optical imaging technique based on gas bubbles induced by combined optical and ultrasound energies

Alternative Title

초음파와 광 에너지를 결합하여 유도된 가스 기포를 기반으로 한 심층 광학 이미징 기술

DGIST Authors

Jinwoo Kim ; Jin Ho Chang ; Jae Youn Hwang

Advisor

장진호

Co-Advisor(s)

Jae Youn Hwang

Issued Date

2025

Awarded Date

2025-02-01

Citation

Jinwoo Kim. (2025). Deep optical imaging technique based on gas bubbles induced by combined optical and ultrasound energies. doi: 10.22677/THESIS.200000839667

Type

Thesis

Description

Optical scattering, Light penetration, Combined ultrasound and laser energy, Gas bubbles, Deep optical microscopy

Table Of Contents

Ⅰ. Introduction 1
1.1 Optical Microscopy 1
1.1.1 Why Optical Microscopy? 1
1.1.2 History and Principle of Optical Microscopy 2
1.2 Light Penetration Depth in Optical Microscopy 6
1.2.1 Interaction in Tissue and Light 8
1.2.2 Limitation of Light Penetration Depth 9
1.3 Deep Tissue Imaging Techniques 14
1.3.1 Two-Photon Microscopy 14
1.3.2 Wavefront Shaping 17
1.3.3 Optical Clearing Methods 21
1.3.4 Ultrasound-Induced Optical Clearing Microscopy (US-OCM) 24
1.4 Objective of Research 27
1.5 Dissertation Organization 28
Reference 30

ⅠI. Control of Optical Imaging Depth using Ultrasound-Induced Gas Bubbles for Deep Optical Microscopy
2.1 Introduction 37
2.2 Principle of FI-OCM 41
2.3 Materials and Methods 43
2.3.1 Configuration of FI-OCM system 43
2.3.2 Fabrication and Characteristics of 2 MHz ring-typed ultrasound transducer with long-wavelength for gas bubble generation. 46
2.3.3 Experimental arrangement for ultrasound-induced gas bubbles generation and observation inside the tissue-mimicking phantom. 49
2.4 Results 51
2.4.1 Observation of the ultrasound-induced gas bubbles due to the change in ultrasound frequency inside the tissue-mimicking phantom. 51
2.4.2 The measurement of light-beam distribution affected by ultrasound-induced gas bubbles by changing the ultrasound frequency. 53
2.4.3 Imaging performance affected by ultrasound-induced gas bubbles by changing the ultrasound frequency in the tissue-mimicking phantom with fluorescent bead. 56
2.5 Experimental Section 59
2.6 Discussion and Conclusion 61
Reference 63

ⅠII. Gas Bubbles Induced by Combined Optical and Ultrasound Energies for High-Resolution Deep Optical Microscopy
3.1 Introduction 67
3.2 Principle of OPS-DOM 71
3.3 Materials and Methods 73
3.3.1 Fabrication of a 1 MHz donut-shaped ultrasound transducer for gas bubble generation 73
3.3.2 Experimental arrangement for optrasound-induced gas bubble generation and observation of the tissue-mimicking phantom 76
3.3.3 OPS-DOM configuration and experimental setup arrangement 79
3.4 Results 81
3.4.1 Parameter determination for pre-established ultrasound field using the finite element method (FEM) 81
3.4.2 Observation of the optrasound-induced gas bubbles inside the tissue-mimicking phantom 83
3.4.3 Measurement of light beam distribution affected by optrasound-induced gas bubbles 91
3.4.4 Imaging performance evaluation of OPS-DOM in the tissue-mimicking phantom 92
3.5 Experimental Section 95
3.6 Discussion and Conclusion 97
Reference 101

IV. In Vivo Study of the Integrated Confocal Fluorescence and Photoacoustic Microscopy for High-Resolution Deep Tissue Imaging
4.1 Introduction 105
4.2 Principle of Multi-OCM 109
4.3 Materials and Methods 112
4.3.1 Configuration of Multi-OCM System 112
4.3.2 Operating sequence of Multi-OCM system 114
4.3.3 Fabrication of a fusion transducer for gas bubble generation and detection of photoacoustic signal 117
4.4 Results 119
4.4.1 Imaging performance evaluation of Multi-OCM in the tissue-mimicking phantom and chicken breast 119
4.4.2 Imaging performance evaluation of Multi-OCM in the mouse tumor for in vivo 123
4.5 Experimental Section 126
4.6 Discussion and Conclusion 129
Reference 132

V. Conclusion and Future Works

URI

http://hdl.handle.net/20.500.11750/57999
http://dgist.dcollection.net/common/orgView/200000839667

DOI

10.22677/THESIS.200000839667

Degree

Doctor

Department

Department of Electrical Engineering and Computer Science

Publisher

DGIST

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