Jinwoo Kim. (2025). Deep optical imaging technique based on gas bubbles induced by combined optical and ultrasound energies. doi: 10.22677/THESIS.200000839667
Type
Thesis
Description
Optical scattering, Light penetration, Combined ultrasound and laser energy, Gas bubbles, Deep optical microscopy
Table Of Contents
Ⅰ. Introduction 1 1.1 Optical Microscopy 1 1.1.1 Why Optical Microscopy? 1 1.1.2 History and Principle of Optical Microscopy 2 1.2 Light Penetration Depth in Optical Microscopy 6 1.2.1 Interaction in Tissue and Light 8 1.2.2 Limitation of Light Penetration Depth 9 1.3 Deep Tissue Imaging Techniques 14 1.3.1 Two-Photon Microscopy 14 1.3.2 Wavefront Shaping 17 1.3.3 Optical Clearing Methods 21 1.3.4 Ultrasound-Induced Optical Clearing Microscopy (US-OCM) 24 1.4 Objective of Research 27 1.5 Dissertation Organization 28 Reference 30
ⅠI. Control of Optical Imaging Depth using Ultrasound-Induced Gas Bubbles for Deep Optical Microscopy 2.1 Introduction 37 2.2 Principle of FI-OCM 41 2.3 Materials and Methods 43 2.3.1 Configuration of FI-OCM system 43 2.3.2 Fabrication and Characteristics of 2 MHz ring-typed ultrasound transducer with long-wavelength for gas bubble generation. 46 2.3.3 Experimental arrangement for ultrasound-induced gas bubbles generation and observation inside the tissue-mimicking phantom. 49 2.4 Results 51 2.4.1 Observation of the ultrasound-induced gas bubbles due to the change in ultrasound frequency inside the tissue-mimicking phantom. 51 2.4.2 The measurement of light-beam distribution affected by ultrasound-induced gas bubbles by changing the ultrasound frequency. 53 2.4.3 Imaging performance affected by ultrasound-induced gas bubbles by changing the ultrasound frequency in the tissue-mimicking phantom with fluorescent bead. 56 2.5 Experimental Section 59 2.6 Discussion and Conclusion 61 Reference 63
ⅠII. Gas Bubbles Induced by Combined Optical and Ultrasound Energies for High-Resolution Deep Optical Microscopy 3.1 Introduction 67 3.2 Principle of OPS-DOM 71 3.3 Materials and Methods 73 3.3.1 Fabrication of a 1 MHz donut-shaped ultrasound transducer for gas bubble generation 73 3.3.2 Experimental arrangement for optrasound-induced gas bubble generation and observation of the tissue-mimicking phantom 76 3.3.3 OPS-DOM configuration and experimental setup arrangement 79 3.4 Results 81 3.4.1 Parameter determination for pre-established ultrasound field using the finite element method (FEM) 81 3.4.2 Observation of the optrasound-induced gas bubbles inside the tissue-mimicking phantom 83 3.4.3 Measurement of light beam distribution affected by optrasound-induced gas bubbles 91 3.4.4 Imaging performance evaluation of OPS-DOM in the tissue-mimicking phantom 92 3.5 Experimental Section 95 3.6 Discussion and Conclusion 97 Reference 101
IV. In Vivo Study of the Integrated Confocal Fluorescence and Photoacoustic Microscopy for High-Resolution Deep Tissue Imaging 4.1 Introduction 105 4.2 Principle of Multi-OCM 109 4.3 Materials and Methods 112 4.3.1 Configuration of Multi-OCM System 112 4.3.2 Operating sequence of Multi-OCM system 114 4.3.3 Fabrication of a fusion transducer for gas bubble generation and detection of photoacoustic signal 117 4.4 Results 119 4.4.1 Imaging performance evaluation of Multi-OCM in the tissue-mimicking phantom and chicken breast 119 4.4.2 Imaging performance evaluation of Multi-OCM in the mouse tumor for in vivo 123 4.5 Experimental Section 126 4.6 Discussion and Conclusion 129 Reference 132
