Dorjrentsen Sugarjav. (2025). Exploring the effect of arginine methylation on RNA-regulated FUS LLPS. doi: 10.22677/THESIS.200000828334
Type
Thesis
Description
FUS, LLPS, RNA, arginine methylation
Table Of Contents
Ⅰ. Introduction 1 Ⅱ. Materials and Methods 5 Ⅲ. Results 10 3.1 Validation of wild-type FUS (FUSWT) fused with fluorescent protein constructs 10 3.2 RNA depletion within FUS granules impairs their ability to assemble in cellular environment 14 3.3 Investigation of the arginine methylation in the RGG1 and RGG2 regions of FUS on its LLPS 17 Ⅳ. Discussion 23 4.1 Cellular stressors and FUS mislocalization 23 4.2 The role of RNA within FUS LLPS in cellular environments 24 4.3 Arginine methylation and its hydrophobicity at the RGG1 and RGG2 regions in FUS-RNA interactions 24 Supplementary information 26 References 33 요약문 (Abstract in Korean) 38
