Title

Exploring the effect of arginine methylation on RNA-regulated FUS LLPS

DGIST Authors

Dorjrentsen Sugarjav ; Jong-Chan Lee ; Byung-Hoon Lee

Advisor

이종찬

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2025

Awarded Date

2025-02-01

Citation

Dorjrentsen Sugarjav. (2025). Exploring the effect of arginine methylation on RNA-regulated FUS LLPS. doi: 10.22677/THESIS.200000828334

Type

Thesis

Description

FUS, LLPS, RNA, arginine methylation

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Materials and Methods 5
Ⅲ. Results 10
3.1 Validation of wild-type FUS (FUSWT) fused with fluorescent protein constructs 10
3.2 RNA depletion within FUS granules impairs their ability to assemble in cellular environment 14
3.3 Investigation of the arginine methylation in the RGG1 and RGG2 regions of FUS on its LLPS 17
Ⅳ. Discussion 23
4.1 Cellular stressors and FUS mislocalization 23
4.2 The role of RNA within FUS LLPS in cellular environments 24
4.3 Arginine methylation and its hydrophobicity at the RGG1 and RGG2 regions in FUS-RNA interactions 24
Supplementary information 26
References 33
요약문 (Abstract in Korean) 38

URI

http://hdl.handle.net/20.500.11750/58012
http://dgist.dcollection.net/common/orgView/200000828334

DOI

10.22677/THESIS.200000828334

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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