Title

Vutiglabridin alleviates cellular senescence phenotypes with enhanced mitochondrial function and metabolic changes

Alternative Title

뷰티글라브리딘은 세포 노화를 감소시키고 미토콘드리아 기능을 증가시키며 노화 세포 내 대사를 변화시킨다

DGIST Authors

Jin-Woong Heo ; Chang-Hoon Nam ; Young-Sam Lee

Advisor

남창훈

Co-Advisor(s)

Young-Sam Lee

Issued Date

2025

Awarded Date

2025-02-01

Citation

Jin-Woong Heo. (2025). Vutiglabridin alleviates cellular senescence phenotypes with enhanced mitochondrial function and metabolic changes. doi: 10.22677/THESIS.200000841459

Type

Thesis

Description

cellular senescence, vutiglabridin, Paraoxonase2, mitochondria, metabolism

Table Of Contents

Ⅰ. Introduction 1


Ⅱ. Materials and Methods 6
2.1 Cell culture 6
2.2 Cell growth assay 6
2.3 Senescence Associated-β-galactosidase (SA-β-gal) assay 7
2.4 RNA extraction and cDNA synthesis 8
2.5 Quantitative real-time PCR 8
2.6 Extracellular Flux assays 9
2.7 Confocal microscopy and Electron microscopy 10
2.8 Western blot 11
2.9 NAD+ and NADH measurement 12
2.10 Cell viability assay 13
2.11 Senescence induction in LO2 cells 14
2.12 generation of PON2 Knock-Down cells 14

Ⅲ. Results 15
3.1 Vutiglabridin treatment alleviates replicative senescence of HDFs 15
3.2 Long-term culture with vutiglabridin reduces mitochondrial dysfunction in high-passage HDFs 20
3.3 Long-term culture with vutiglabridin modulates the expression of the metabolic regulatory protein in high-passage HDFs 27
3.4 Treatment of low concentration hydrogen peroxide provokes ROS-induced senescence in L02 hepatocytes 30
3.5 Vutiglabridin alleviates ROS-induced senescence in LO2s with mitochondria morphological change 35

Ⅳ. Discussion 41

Ⅴ. Reference 44

URI

http://hdl.handle.net/20.500.11750/58015
http://dgist.dcollection.net/common/orgView/200000841459

DOI

10.22677/THESIS.200000841459

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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