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Odorant Receptors Containing Conserved Amino Acid Sequences in Transmembrane Domain 7 Display Distinct Expression Patterns in Mammalian Tissues

Title
Odorant Receptors Containing Conserved Amino Acid Sequences in Transmembrane Domain 7 Display Distinct Expression Patterns in Mammalian Tissues
Authors
Ryu, Sang EunShim, TammyYi, Ju YeonKim, So YeonPark, Sun HwaKim, Sung WonRonnett, Gabriele V.Moon, Cheil
DGIST Authors
Moon, Cheil
Issue Date
2017-12
Citation
Molecules and Cells, 40(12), 954-965
Type
Article
Article Type
Article
Keywords
PROTEIN-COUPLED RECEPTORMOUSE OLFACTORY SYSTEMHUMAN SPERMCELLSBRAINBULBIDENTIFICATIONRECOGNITIONACTIVATIONEPITHELIUM
ISSN
1016-8478
Abstract
Mammalian genomes are well established, and highly conserved regions within odorant receptors that are unique from other G-protein coupled receptors have been identified. Numerous functional studies have focused on specific conserved amino acids motifs; however, not all conserved motifs have been sufficiently characterized. Here, we identified a highly conserved 18 amino acid sequence motif within transmembrane domain seven (CAS-TM7) which was identified by aligning odorant receptor sequences. Next, we investigated the expression pattern and distribution of this conserved amino acid motif among a broad range of odorant receptors. To examine the localization of odorant receptor proteins, we used a sequence-specific peptide antibody against CAS-TM7 which is specific to odorant receptors across species. The specificity of this peptide antibody in recognizing odorant receptors has been confirmed in a heterologous in vitro system and a rat-based in vivo system. The CAS-TM7 odorant receptors localized with distinct patterns at each region of the olfactory epithelium; septum, endoturbinate and ectoturbinate. To our great interests, we found that the CAS-TM7 odorant receptors are primarily localized to the dorsal region of the olfactory bulb, coinciding with olfactory epithelium-based patterns. Also, these odorant receptors were ectopically expressed in the various non-olfactory tissues in an evolutionary constrained manner between human and rats. This study has characterized the expression patterns of odorant receptors containing particular amino acid motif in transmembrane domain 7, and which led to an intriguing possibility that the conserved motif of odorant receptors can play critical roles in other physiological functions as well as olfaction.
URI
http://hdl.handle.net/20.500.11750/5808
DOI
10.14348/molcells.2017.0223
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Related Researcher
  • Author Moon, Cheil Moon Lab
  • Research Interests Brain convergent science based on chemical senses; olfaction; 감각신경계 기반 뇌융합과학; 후각 신경계
Files:
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Collection:
Department of Brain and Cognitive SciencesMoon Lab1. Journal Articles


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