An overview of label-free quantitative holographic live cell imaging for drug toxicity assessment

Abstract

Quantitative phase imaging with digital holographic microscopy (QP-DHM) can be employed to quantify the movement of cardiomyocytes. In this paper, we review the application of QP-DHM in studying cardiomyocytes derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. This allowed us to extract a set of several parameters that can characterize the cardiomyocytes beating patterns from the recorded quantitative phase signal. We monitored the effects of E-4031 and isoprenaline on the cardiomyocyte beating patterns. Our results show that some effects specific to the mode of action of the drugs used can be identified. This stresses that QP-DHM can represent a promising label-free approach to identify new drug candidates by measuring their effects on iPSC-derived cardiomyocytes. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.